1887

Abstract

Summary: The lipopolysaccharide of free-living was isolated and purified, and its homogeneity was determined by gel electrophoresis and, after mild acid degradation, by gel filtration. On electrophoresis, two molecular species were observed. After acid degradation and gel filtration, three components could be isolated: one was very rich in glucose, one contained 2--methylfucose, fucose, mannose, galactose, glucose, ----heptose, 2-keto-3-deoxyoctonic acid, -alanine, quinovosamine and uronic acids, and the third component consisted of low molecular weight material. In the lipid A fraction, -glucosamine, β-hydroxymyristic acid, β-hydroxypalmitic acid and β-hydroxystearic acid were detected as major components. The phosphorus content was low. No major chemical differences were observed in the neutral sugar and fatty acid compositions of the lipopolysaccharides isolated from bacteria and bacteroids. The lipopolysaccharide of bacteroids was rapidly lost during isolation from the nodules and on dialysis, and behaved anomalously during ultracentrifugation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-110-1-151
1979-01-01
2021-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/110/1/mic-110-1-151.html?itemId=/content/journal/micro/10.1099/00221287-110-1-151&mimeType=html&fmt=ahah

References

  1. Aminoff D., Morgan W. T. J. 1948; Hexosamine components of the human blood group substances.. Nature, London 162:579–580
    [Google Scholar]
  2. Bauer W. D. 1977; Lectins as determinants of specificity in legume–Rhizobium symbiosis.. Basic Life Sciences 9:283–298
    [Google Scholar]
  3. Boivin A., Mesrobeanu L. 1935; Recherches sur les antigènes somatiques et sur les endotoxines des bactéries. Considérations générates et exposé des techniques utilisées.. Review of Immunology 1:553–569
    [Google Scholar]
  4. Brossmer R. 1962; 2-Amino-2-deoxy-d-galactose hydrochloride.. Methods in Carbohydrate Chemistry 1:216–221
    [Google Scholar]
  5. Bryson J. L., Mitchell T. J. 1951; Improved spraying reagents for the detection of sugars on paper chromatograms.. Nature, London 167:864
    [Google Scholar]
  6. Crumpton M.J. 1959; Identification of amino-sugars.. Biochemical Journal 72:479–486
    [Google Scholar]
  7. Dische Z. 1947; A new specific color reaction of hexuronic acids.. Journal of Biological Chemistry 167:189–198
    [Google Scholar]
  8. Edwards J. T., Waldron D. M. 1952; Detection of deoxy sugars, glycols and methylpentoses in paper partition chromatography.. Journal of the Chemical Society3631–3634
    [Google Scholar]
  9. Folch J., Lees M., Sloane-Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues.. Journal of Biological Chemistry 226:497–509
    [Google Scholar]
  10. Galanos C., Lüderitz O., Westphal O. 1969; A new method for the extraction of R lipopolysaccharides.. European Journal of Biochemistry 9:245–249
    [Google Scholar]
  11. Graham P. H., O’Brien M. A. 1968; Composition of lipopolysaccharides from Rhizobium and Agrobacterium. Antonie van Leeuwenhoek 34:326–330
    [Google Scholar]
  12. Hase S., Rietschel E. T. 1976; Isolation and analysis of the lipid A backbone. Lipid A structure of lipopolysaccharides from various bacterial groups.. European Journal of Biochemistry 63:101–107
    [Google Scholar]
  13. Humphrey B. A., Vincent J. M. 1969; The somatic antigens of two strains of Rhizobium trifolii. Journal of General Microbiology 59:411–425
    [Google Scholar]
  14. Jann B., Reske K., Jann K. 1975; Heterogeneity of lipopolysaccharide chain lengths by sodium dodecylsulphate polyacrylamide gel electrophoresis.. European Journal of Biochemistry 60:239–246
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Lowry O. H., Roberts N. R., Leiner K. Y., Wu M. L., Farr A. L. 1954; The quantitative histochemistry of brain. I. Chemical methods.. Journal of Biological Chemistry 207:1–17
    [Google Scholar]
  17. McOmie J. F. W., Watts M. L., West D. E. 1968; Demethylation of acrylmethyl ethers by borontribromide.. Tetrahedron 24:2289–2292
    [Google Scholar]
  18. Oparin A. 1976; Chemical analysis of the lipopolysaccharide of bacteroids of Rhizobium lupini. Proceedings of the National Academy of Sciences (USSR) 231:1482–1485
    [Google Scholar]
  19. Osborn M. J., Gander J. E., Parisi E., Carson J. 1972; Mechanism and assembly of the outer membrane of Salmonella typhimurium. Journal of Biological Chemistry 247:3962–3972
    [Google Scholar]
  20. Partridge S. M., Westall R. G. 1948; Filter paper partition chromatography of sugars. I. Qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep.. Biochemical Journal 42:238–250
    [Google Scholar]
  21. Planqué K., Kijne J. W. 1977; Binding of pea lectins to a glycan type polysaccharide in the cell walls of Rhizobium leguminosarum. FEBS Letters 73:64–66
    [Google Scholar]
  22. Planqué K., van Brussel A. A. N. 1976; An improved large scale isolation procedure for bacteroids of Rhizobium leguminosarum Frank, from Pisum sativum. Plant and Soil 45:309–315
    [Google Scholar]
  23. Planqué K., Kennedy I. R., de Vries G. E., Quispel A., van Brussel A. A. N. 1977; Location of nitrogenase and ammonia-assimila-tory enzymes in bacteroids of Rhizobium leguminosarum and Rhizobium lupini. Journal of General Microbiology 102:95–104
    [Google Scholar]
  24. Rondle C. J. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine.. Biochemical Journal 61:586–589
    [Google Scholar]
  25. Russa R., Lorkiewicz Z. 1974; Fatty acids present in the lipopolysaccharide of Rhizobium trifolii. Journal of Bacteriology 119:771–775
    [Google Scholar]
  26. Salkinoja-Salonen M., Boeck R. 1978; Characterization of lipopolysaccharides isolated from Agrobacterium tumefaciens. Journal of General Microbiology 105:119–125
    [Google Scholar]
  27. Sanders R. E., Carlson W. R., Albersheim P. 1978; A Rhizobium mutant incapable of nodulation and normal polysaccharide secretion.. Nature, London 271:240–242
    [Google Scholar]
  28. Sawardekar S., Sloneker J. H., Jeanes A. R. 1965; The detection of monosaccharides and their acetates by gas–liquid chromatography.. Analytical Chemistry 37:1602–1604
    [Google Scholar]
  29. Schmidt G., Fromme I., Mayer H. 1970; Immunochemical studies on core lipopolysaccharides of Enterobacteriaceae of different genera.. European Journal of Biochemistry 14:357–366
    [Google Scholar]
  30. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; The detection of sugars on paper chromatograms.. Nature, London 166:444–445
    [Google Scholar]
  31. Van Brussel A. A. N., Planqué K., Quispel A. 1977; The wall of Rhizobium leguminosarum in bacteroids and free-living forms.. Journal of General Microbiology 101:51–56
    [Google Scholar]
  32. Weckesser J., Mayer H., Drews G., Fromme I. 1975; Low-molecular-weight polysaccharide antigens isolated from Rhodopseudomonas gelatinosa. Journal of Bacteriology 123:456–462
    [Google Scholar]
  33. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure.. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
  34. Wilkinson S. 1976; Amino components of the lipopolysaccharide from Pseudomonas aeruginosa N.C.T.C. 8505.. Biochemical Journal 161:103–109
    [Google Scholar]
  35. Yoshida A. 1965; Micromethod for the quantitative determination of l-alanine.. Analytical Biochemistry 11:383–386
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-110-1-151
Loading
/content/journal/micro/10.1099/00221287-110-1-151
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error