Summary: Mutants deficient in several enzymes of methylamine oxidation and assimilation have been obtained by treating with ultraviolet light. One of these mutants was studied in detail and was shown to lack the enzymes of trimethylamine and dimethylamine oxidation. In addition, activities of the following enzymes, all postulated as being involved in C metabolism, were lost: hydroxypyruvate reductase, serine-glyoxylate aminotransferase, isocitrate lyase, phospho/pyruvate carboxylase, -methylglutamate dehydrogenase, γ-glutamylmethylamide synthetase, formate dehydrogenase and dye-linked formaldehyde dehydrogenase. In contrast, three enzymes - NAD,glutathione-linked formaldehyde dehydrogenase, -methylalanine dehydrogenase and -methylglutamate synthase - were retained. This phenotype suggests either a lesion in a regulatory gene, a deletion in an early structural gene of an operon or the loss of a plasmid. Results obtained indicated that possesses an isocitrate lyase-serine pathway.


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