1887

Abstract

Summary: Intracellular ATP concentrations and energy charge were monitored during the growth of and related to the number of colony-forming units, to the pH of the medium and to incorporation of radioactive precursors. Of three different methods used for extracting nucleotides - trichloroacetic acid, perchloric acid and boiling Tris/HSO buffer - the trichloroacetic acid treatment was the most effective. During the active growth phase the ATP concentration increased exponentially, in the same way as other growth parameters. However, as soon as the metabolic activity slowed down, there was a rapid drop of ATP concentration and of total adenylate content, which was followed much later by a parallel decrease in the number of colony-forming units. During active growth, energy charge remained at around 0·9 and decreased only slowly during the stationary phase. Incorporation of labelled thymidine or phenylalanine followed a different pattern during the late phase of the culture and gave less information concerning the viability of the organisms.

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1979-01-01
2024-04-23
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References

  1. Atkinson D. E. 1968; The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers.. Biochemistry 7:4030–4034
    [Google Scholar]
  2. Atkinson D. E. 1977; Adenylate energy charge is a key factor.. Trends in Biochemical Sciences 2:N198–N200
    [Google Scholar]
  3. Ball W. J. Jr, Atkinson D. E. 1975; Adenylate energy charge in Saccharomyces cerevisiae during starvation.. Journal of Bacteriology 121:975–982
    [Google Scholar]
  4. Bomsel J. L., Pradet A. 1968; Study of adenosine-5ʹ-mono, di and triphosphate in plant tissues. IV. Regulation of the level of nucleotides, in vivo, by adenylate kinase: theoretical and experimental studies.. Biochimica et biophysica acta 162:230–242
    [Google Scholar]
  5. Chapman A. G., Atkinson D. E. 1973; Stabilization of adenylate energy charge by the adenylate deaminase reaction.. Journal of Biological Chemistry 248:8309–8312
    [Google Scholar]
  6. Chapman A. G., Fall L., Atkinson D. E. 1971; Adenylate energy charge in Escherichia coli during growth and starvation.. Journal of Bacteriology 108:1072–1085
    [Google Scholar]
  7. Daniels M. J., Markham P. G., Meddins B. M., Plaskitt A. K., Townsend R., Bar-Joseph M. 1973; Axenic culture of a plant pathogenic spiroplasma.. Nature, London 244:523–524
    [Google Scholar]
  8. Edwards S. W., Lloyd D. 1977; Changes in oxygen uptake rates, enzyme activities, cytochrome amounts and adenine nucleotide pool levels during growth of Acanthamoeba castellanii in batch cultures.. Journal of General Microbiology 102:135–144
    [Google Scholar]
  9. Edwards C., Statham M., Lloyd D. 1975; The preparation of large-scale synchronous cultures of the trypanosomatid, Crithidia fasciculata, by cell-size selection: changes in respiration and adenylate charge through the cell-cycle.. Journal of General Microbiology 88:141–152
    [Google Scholar]
  10. Fromm H. 1977; Control by energy charge is an untenable theory.. Trends in Biochemical Sciences 2:N198–N200
    [Google Scholar]
  11. Holm-Hansen O. 1969; Determination of microbial biomass in ocean profiles.. Limnology and Oceanography 14:740–747
    [Google Scholar]
  12. Kimmich G. A., Randles J., Brand J. S. 1975; Assay of picomole amounts of ATP, ADP, and AMP using the luciferase enzyme system.. Analytical Biochemistry 69:167–206
    [Google Scholar]
  13. Knowles C. J. 1977; Microbial metabolic regulation by adenine nucleotide pools.. Symposia of the Society for General Microbiology 27:241–283
    [Google Scholar]
  14. Lloyd D., Phillips C. A., Statham M. 1978; Oscillations of respiration, adenine nucleotide levels and heat evolution in synchronous cultures of Tetrahymena pyriformis st prepared by continuous-flow selection.. Journal of General Microbiology 106:19–26
    [Google Scholar]
  15. MacLeold N. H., Chapelle E. W., Crawford A. M. 1969; ATP assay of terrestrial soils: a test of an exbiological experiment.. Nature, London 223:267–268
    [Google Scholar]
  16. Niven D. F., Collins P. A., Knowles C. J. 1977; Adenylate energy charge during batch culture of Beneckea natriegens. Journal of General Microbiology 98:95–108
    [Google Scholar]
  17. Pradet A. 1967; Étude des adenosines-5ʹ-mono, di et triphosphates dans les tissues végétaux. I. Dosage enzymatique.. Physiologie Végétale 5:209–221
    [Google Scholar]
  18. Saglio P., L’Hospital M., Lafleche D., Dupont G., Bove J. M., Tully J. G., Freundt E. A. 1973; Spiroplasma citri gen. and sp.n.: a mycoplasma-like organism associated with ‘stubborn’ disease of citrus.. International Journal of Systematic Bacteriology 23:191–204
    [Google Scholar]
  19. Saglio P., Daniels M. J., Pradet A. 1976; ATP and energy charge as criteria of growth and metabolic activity of Mollicutes: application to Spiroplasma citri. Proceedings of the Society for General Microbiology 3:155
    [Google Scholar]
  20. Schram E. 1970; Use of scintillation counters for bioluminescence assay of adenosine triphosphate (ATP). In The Current Status of Liquid Scintillation Counting p. 129 Edited by Bransome E. D. Jr. New York: Grune and Stratton;
    [Google Scholar]
  21. Schramm V. L., Leung H. 1973; Regulation of adenosine monophosphate levels as a function of adenosine triphosphate and inorganic phosphate. A proposed metabolic role for adenosine monophosphate nucleosidase from Azotobacter vinelandii. Journal of Biological Chemistry 248:8313–8315
    [Google Scholar]
  22. Windsor R. S., Boarer C. D. H. 1972; A method for rapid enumeration of mycoplasma species growing in broth culture.. Journal of Applied Bacteriology 35:37–42
    [Google Scholar]
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