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SUMMARY: Further recombination data have been obtained from matings involving different F+ auxotrophic mutants of Escherichia coli strain K-12 with the same K-12 F− auxotroph. Variations in the distribution of sugar fermentation markers among the progeny have been explained by the influence of the ‘contra-selected’ F+ auxotrophic marker. It is suggested that the data supports the conception of a single linear chromosome in K-12, which fragments randomly during recombination.
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