1887

Abstract

SUMMARY: Two strains of , which require α-amino nitrogen for growth as a result of a single gene mutation (), lack glutamic dehydrogenase. Extracts of mutant mycelium contained no detectable enzyme, although as little as 0·2 % of the amount normally present in the wild type should have been detectable in some experiments. Mutant extracts contained no detectable enzyme inhibitor. A balanced heterocaryon containing nuclei and nuclei carrying an unrelated mutation produced less enzyme than did wild type mycelium, but there was no indication that the nuclei were suppressing the activity of the non- nuclei in promoting enzyme production. The production of the enzyme by the wild type was not strikingly dependent on conditions of growth although the addition of glutamate to the medium seemed somewhat to depress the enzyme concentration. This effect was more marked when glutamate was supplied as a sole nitrogen source. The mutation had little or no effect on the concentrations of two other dehydrogenases or of several transaminases, all related to glutamic dehydrogenase in their substrate specificities. The possible significance of these observations is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-11-2-236
1954-10-01
2021-07-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/11/2/mic-11-2-236.html?itemId=/content/journal/micro/10.1099/00221287-11-2-236&mimeType=html&fmt=ahah

References

  1. Cohn M., Monod J. 1953; Specific induction and inhibition of enzyme biosynthesis. In Adaptation in Micro-organisms. Symp. Soc. gen. Microbiol 3:132
    [Google Scholar]
  2. Ephrussi B. 1953 Nucleo-Cytoplasmic Relations in Micro-organisms Oxford: Clarendon Press;
    [Google Scholar]
  3. Evans H. J., Nason A. 1953; Pyridine nucleotide-nitrate reductase from extracts of higher plants. Plant Physiol 28:233
    [Google Scholar]
  4. Fincham J. R. S. 1950; Mutant strains of Neurospora deficient in animating ability. J. biol. Chem 182:61
    [Google Scholar]
  5. Fincham J. R. S. 1951a; The occurrence of glutamic dehydrogenase in Neurospora and its apparent absence in certain mutant strains. J. gen. Microbiol 5:793
    [Google Scholar]
  6. Fincham J. R. S. 1951b; Transaminases in Neurospora crassa. Nature; Lond: 168957
    [Google Scholar]
  7. Fincham J. R. S. 1953; Ornithine transaminase in Neurospora and its relation to the biosynthesis of proline. Biochem J 53:313
    [Google Scholar]
  8. Fowden L. 1951; The quantitative recovery and colorimetric estimation of amino acids separated by chromatography. Biochem. J 48:327
    [Google Scholar]
  9. LePage G. A., Mueller G. C. 1949; Preparation of TPN. J. biol. Chem 180:975
    [Google Scholar]
  10. Muller H. J. 1932; Further studies on the nature and causes of gene mutation. Proc. 6th Int. Conf. Genet 1:213
    [Google Scholar]
  11. Ryan F. J., Beadle G. W., Tatum E. L. 1943; The tube method of measuring the growth rate of Neurospora. Amer. J. Bot 30:784
    [Google Scholar]
  12. Stickland L. H. 1951; The determination of small quantities of bacteria by means of the biuret reaction. J. gen. Microbiol 5:698
    [Google Scholar]
  13. Tissiéres A., Mitchell H. K., Haskins F. A. 1953; Studies on the respiratory system of the poky strains of Neurospora. J. biol. Chem 205:423
    [Google Scholar]
  14. Umbreit W. W., Burris R. H., Stauffer J. F. 1949 Manometric Methods and Tissue Metabolism Minneapolis: Burgess Publishing Co;
    [Google Scholar]
  15. Westergaard M., Mitchell H. K. 1947; Neurospora. V. A. synthetic medium favouring sexual reproduction. Amer. J. Bot 34:573
    [Google Scholar]
  16. Yanofsky C. 1953; Further studies with the td mutants of Neurospora. Genetics 38:702 Abstr
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-11-2-236
Loading
/content/journal/micro/10.1099/00221287-11-2-236
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error