pH Stability and Purification of Lumpy Skin Disease Virus

SUMMARY: Lumpy skin disease virus in allantoic fluid was found to be stable when subjected to wide variation of hydrogen-ion concentration under differing conditions of time and temperature. The virus could be concentrated by adsorption on the precipitate formed when allantoic fluid was dialysed at pH 4.5; adsorption on calcium phosphate was also demonstrated. Preliminary purification of the virus could be effected by these methods.