1887

Abstract

SUMMARY: A number of cultures of chitin-decomposing aerobic bacteria were isolated by the enrichment technique. Chitin decomposition was followed by measurement of loss of alkali-resistant insoluble substrate. Agitated submerged cultures secured a much more rapid breakdown of chitin than static cultures. The exocellular chitinase produced by a species of in agitated submerged culture was studied. The effects of pH value and substrate concentration on enzyme activity were examined and the results used to establish a chitinase assay in terms of reducing-sugar production. The enzyme was an inducible one (adaptive). The enzyme was concentrated by ultrafiltration and lyophilization. The water-soluble reducing materials produced by the enzyme from chitin were identified as -acetylglucosamine and its corresponding disaccharide, .-diacetylchitobiose.

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/content/journal/micro/10.1099/00221287-11-2-150
1954-10-01
2022-08-14
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References

  1. Bates F. et al. 1942 Polarimetry, Saccharimetry, and the Sugars Washington: U.S Government Printing Office;
    [Google Scholar]
  2. Benecke W. 1905; Über Bacillus chitinovorus, einen Chitin zersetzenden Spaltpilz. Bot. Ztg 1. Abt 63:227
    [Google Scholar]
  3. Bergmann M., Zervas L., Silberkweit E. 1931; Über Chitin und Chitobiose. Ber. dtsch. chem. Ges 64:2436
    [Google Scholar]
  4. Bucherer H. 1935; Über den mikrobiellen Chitinabbau. Zbl. Bakt 2. Abt 93:12
    [Google Scholar]
  5. Chargaff E., Levine C., Green C. 1948; Techniques for the demonstration by chromatography of nitrogenous lipid constituents, sulfur-containing amino acids, and reducing sugars. J. biol. Chem 175:67
    [Google Scholar]
  6. Folpmers T. 1921; De ontleding van chitine en zijn splitsingsproduct, het glucosamine, door bacteriën. Chem. Weekbl 18:249
    [Google Scholar]
  7. Grassmann W., Rubenbauer H. 1931; Über Zellulase und Hemizellulase mit besonderer Berücksichtigung ihrer therapeutischen Anwendung. Münch, med. Wschr 78:1817
    [Google Scholar]
  8. Jeuniaux C. 1950; Production d’une exochitinase par des bactéries chitinolytiques isolées du contenu intestinal de l’escargot. Arch. int. Physiol 58:352
    [Google Scholar]
  9. Jeuniaux C. 1951; Une méthode de dosage des chitinases. Arch. int. Physiol 59:242
    [Google Scholar]
  10. Kabat E. A., Mayer M. M. 1948 Experimental Immunochemistry Springfield Ill: Chas. C. Thomas;
    [Google Scholar]
  11. Karrer P., Hofmann A. 1929; Polysaccharide XXXIX. Über den enzy-matischen Abbau von Chitin und Chitosan 1. Helv. Chim. Acta 12:616
    [Google Scholar]
  12. Levinson H. S., Mandels G. R., Reese E. T. 1951; Products of enzymatic hydrolysis of cellulose and its derivatives. Arch. Biochem. Biophys 31:351
    [Google Scholar]
  13. Reese E. T. 1947; On the effect of aeration and nutrition on cellulose decomposition by certain bacteria. J. Bact 53:389
    [Google Scholar]
  14. Sørensen H. 1952; On the specificity and products of action of xylanase from Chaetomium globosum Kunze. Physiol. Plant 5:183
    [Google Scholar]
  15. Sumner J. B., Somers G. F. 1949 Laboratory Experiments in Biological Chemistry, 2nd ed. New York: Academic Press;
    [Google Scholar]
  16. Whistler R. L., Smart C. L. 1953; Isolation of crystalline d-glucose and cello-biose from an enzymatic hydrolyzate of cellulose. J. Amer. Chem. Soc 75:1916
    [Google Scholar]
  17. Zechmeister L., Tóth G. 1931; Zur Kenntnis der Hydrolyse von Chitin mit Salzsaure (1. Mitteil.). Ber. dtsch. chem. Ges 64:2028
    [Google Scholar]
  18. Zechmeister L., Tóth G. 1939; Chromatographische Zerlegung der Chitinase. Naturwissenschaften 27:367
    [Google Scholar]
  19. Zechmeister L., Tóth G., Balint M. 1938; Über die chromatographische Trennung einiger Enzyme des Emulsins. Enzymologia 5:302
    [Google Scholar]
  20. ZoBell C. E., Rittenberg S. C. 1938; The occurrence and characteristics of chitinoclastic bacteria in the sea. J. Bact 35:275
    [Google Scholar]
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