1887

Abstract

SUMMARY: A number of cultures of chitin-decomposing aerobic bacteria were isolated by the enrichment technique. Chitin decomposition was followed by measurement of loss of alkali-resistant insoluble substrate. Agitated submerged cultures secured a much more rapid breakdown of chitin than static cultures. The exocellular chitinase produced by a species of in agitated submerged culture was studied. The effects of pH value and substrate concentration on enzyme activity were examined and the results used to establish a chitinase assay in terms of reducing-sugar production. The enzyme was an inducible one (adaptive). The enzyme was concentrated by ultrafiltration and lyophilization. The water-soluble reducing materials produced by the enzyme from chitin were identified as -acetylglucosamine and its corresponding disaccharide, .-diacetylchitobiose.

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/content/journal/micro/10.1099/00221287-11-2-150
1954-10-01
2019-10-18
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