SUMMARY: A number of cultures of chitin-decomposing aerobic bacteria were isolated by the enrichment technique. Chitin decomposition was followed by measurement of loss of alkali-resistant insoluble substrate. Agitated submerged cultures secured a much more rapid breakdown of chitin than static cultures. The exocellular chitinase produced by a species of in agitated submerged culture was studied. The effects of pH value and substrate concentration on enzyme activity were examined and the results used to establish a chitinase assay in terms of reducing-sugar production. The enzyme was an inducible one (adaptive). The enzyme was concentrated by ultrafiltration and lyophilization. The water-soluble reducing materials produced by the enzyme from chitin were identified as -acetylglucosamine and its corresponding disaccharide, .-diacetylchitobiose.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error