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The chromosomal location of a host gene, mop (groE), which is essential for the morphogenesis of several bacteriophages in Escherichia coli, was determined by two- and three-factor transductional crosses using phage P1. Cotransduction frequencies between mop and other markers were: aspA, 90%; ampA, 77%; frdA, 73%; mel, 24%. The sequence of markers in the corresponding segment (mel to purA; 91·5 to 93·5 min) of the E. coli linkage map was shown to be mel–aspA–mop(groE)–ampA–frdA–purA.
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