1887

Abstract

Conidia of had a very low activity of a high affinity ammonia transport system. During germination the activity increased, reaching a maximum soon after the onset of exponential growth. The activity in germinated conidia varied with the nitrogen source in the germination medium. Analyses of intracellular ammonia and amino acids revealed that a low level of ammonia transport activity was associated with a high intracellular concentration of glutamine and . The intracellular concentration of glutamine is probably involved in repression of synthesis of the ammonia transport system. It is suggested that glutamine and asparagine (rather than ammonia itself) regulate the pre-formed ammonia transport system by inhibition from within the intracellular pool. It is concluded that glutamine and glutamine synthetase may be more important in the regulation of some aspects of nitrogen metabolism rather than ammonia and glutamate dehydrogenase as has been previously suggested.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-109-2-275
1978-12-01
2021-05-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/109/2/mic-109-2-275.html?itemId=/content/journal/micro/10.1099/00221287-109-2-275&mimeType=html&fmt=ahah

References

  1. Anthony C., Cook R. J. 1973; Regulation of methylamine transport during germination of Aspergillus nidulans . Journal of General Microbiology 77:vii
    [Google Scholar]
  2. Cook R. J., Anthony C. 1978; The ammonia and methylamine active transport system of Aspergillus nidulans . Journal of General Microbiology 109:265–274
    [Google Scholar]
  3. Hackette S. L., Skye G. E., Burton C., Segal I. H. 1970; Characterization of an ammonium transport system in filamentous fungi with methylammonium-14C as the substrate. Journal of Biological Chemistry 245:4241–4250
    [Google Scholar]
  4. Hynes M. J. 1973; Pleiotropic mutants affecting the control of nitrogen metabolism in Aspergillus nidulans . Molecular and General Genetics 125:99–107
    [Google Scholar]
  5. Meyers E., Knight S. G. 1953; Studies on the intracellularamino acids of Penicillium roqueforti . Mycologia 53:115–122
    [Google Scholar]
  6. Pall M. L. 1970; Amino acid transport in Neurospora crassa II. Properties of a basic amino acid transport system. Biochimica et biophysica acta 203:473–483
    [Google Scholar]
  7. Pateman J. A., Kinghorn J. R., Dunn E., Forbes E. 1973; Ammonium regulation in Aspergillus nidulans . Journal of Bacteriology 114:943–950
    [Google Scholar]
  8. Robinson J. H., Anthony C., Drabble W. T. 1973a; The acidic amino acid permease of Aspergillus nidulans . Journal of General Microbiology 79:53–63
    [Google Scholar]
  9. Robinson J. H., Anthony C., Drabble W. T. 1973b; Regulation of the acidic amino acid permease of Aspergillus nidulans . Journal of General Microbiology 79:65–80
    [Google Scholar]
  10. Tisdale J. H., DeBusk A. G. 1972; Permeability problems encountered when treating conidia of Neurospora crassa with RNA synthesis inhibitors. Biochemical and Biophysical Research Communications 48:812–822
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-109-2-275
Loading
/content/journal/micro/10.1099/00221287-109-2-275
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error