Summary: Conidia of germinated on glutamate have a single system for the active transport of ammonia and methylamine against a concentration gradient. The transport of methylamine is inhibited by azide, -ethylmaleimide, valinomycin and anaerobiosis; it has a pH optimum of about 6·3 and a temperature optimum of 40°C. The affinity of the system for methylamine (K 13 μ) is about 20% of that for ammonia; the V is also much lower. This transport system is present in methylamine-resistant mutants ( and loci) and its formation is repressed in wild type and mutants by ammonia or a metabolite of ammonia. It is proposed that the system functions in the scavenging of low concentrations of ammonia during nitrogen starvation or during growth on other major nitrogen sources.


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