@article{mbs:/content/journal/micro/10.1099/00221287-109-2-237, author = "Engels, W. and Kamps, M. and Van Boven, C. P. A.", title = "Influence of Cultivation Conditions on the Production of Staphylocoagulase by Staphylococcus aureus 104", journal= "Microbiology", year = "1978", volume = "109", number = "2", pages = "237-243", doi = "https://doi.org/10.1099/00221287-109-2-237", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-109-2-237", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "High yields of staphylocoagulase from Staphylococcus aureus 104 were obtained in a simple salts medium supplemented with glycerol, casein hydrolysate and three vitamins. Conditions of oxygen-limitation (dissolved oxygen concentration ≪2%), a pH of 7·4, a temperature of 35°C and a 1 in 10 inoculum of overnight culture were required for optimal yields of staphylocoagulase.", }