1887

Abstract

High yields of staphylocoagulase from 104 were obtained in a simple salts medium supplemented with glycerol, casein hydrolysate and three vitamins. Conditions of oxygen-limitation (dissolved oxygen concentration ≪2%), a pH of 7·4, a temperature of 35°C and a 1 in 10 inoculum of overnight culture were required for optimal yields of staphylocoagulase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-109-2-237
1978-12-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/109/2/mic-109-2-237.html?itemId=/content/journal/micro/10.1099/00221287-109-2-237&mimeType=html&fmt=ahah

References

  1. Altenbern R. A. 1966; On the nature of albumin-promoted coagulase release by Staphylococcus aureus . Journal of Infectious Diseases 116:593–600
    [Google Scholar]
  2. Arvidson S., Bjorklind A., Eriksson R., Holme T. 1976; Enzymes from Staphylococcus aureus . Continuous Culture: Applications and New Fields 6238–250 Dean A. C. R., Ellwood D. C., Evans C. G. T., Melting J. Chichester: Ellis Horwood;
    [Google Scholar]
  3. Bas B. M., Muller A. D., Hemker H. C. 1974; Purification and properties of staphylocoagulase. Biochimica et biophysica acta 379:164–171
    [Google Scholar]
  4. Blobel H., Berman D. T. 1960; Serologic studies of purified staphylococcal coagulase with special reference to fluorescence microscopy. Journal of Immunology 85:244–249
    [Google Scholar]
  5. Blobel H., Berman D. T., Simon J. 1960; Purification of staphylococcal coagulase. Journal of Bacteriology 79:807–815
    [Google Scholar]
  6. Carpenter D. F., Silverman G. J. 1976; Synthesis of staphylococcal enterotoxin A and nuclease under controlled fermentor conditions. Applied and Environmental Microbiology 31:243–248
    [Google Scholar]
  7. Cooper C. M., Fernstrom G. A., Miller S. A. 1944; Performance of agitated gas-liquid contactors. Industrial and Engineering Chemistry 36:504–509
    [Google Scholar]
  8. Duthie E. S., Haughton G. 1958; Purification of free staphylococcal coagulase. Biochemical Journal 70:125–134
    [Google Scholar]
  9. Evans C. G. T., Herbert D., Tempest D. W. 1970; The continuous cultivation of microorganisms. II. Construction of a chemostat. Methods in Microbiology 2:277–328
    [Google Scholar]
  10. Fahlberg W. J., Marston J. 1960; Coagulase production by Staphylococcus aureus I. Factors influencing coagulase production. Journal of Infectious Diseases 106:111–115
    [Google Scholar]
  11. Hemker H. C., Bas B. M., Muller A. D. 1975; Activation of a pro-enzyme by a stoichiometric reaction with another protein. The reaction between prothrombin and staphylocoagulase. Biochimica et biophysica acta 379:180–188
    [Google Scholar]
  12. Jacherts D. 1956; Zur Reindarstellung der Staphylokokken Coagulase. Zeitschrift für Hygiene, Medizinische Mikrobiologie, Immunologie und Virologie 142:213–218
    [Google Scholar]
  13. Jeljaszewicz J. 1958; Studies on staphylococcal coagulases. Acta microbiologica polonia 7:17–34
    [Google Scholar]
  14. Marston J., Fahlberg J. 1960; Coagulase production by Staphylococcus aureus II. Growth and coagulase production in complex and chemically defined mediums - comparison of chemically defined mediums. Journal of Infectious Diseases 106:116–122
    [Google Scholar]
  15. Murray M., Ghodes P. 1960; Purification of the staphylococcal coagulase. Biochimica et biophysica acta 40:518–522
    [Google Scholar]
  16. Siwecka M., Jeljaszewicz J. 1968; Purification of staphylocoagulase and some of its properties. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originale A) 208:385–394
    [Google Scholar]
  17. Soulier J. P., Lewi S., Panty A. M., Prou-Wartelle O. 1967; Préparation, concentration et étalonnage de la staphylocoagulase. Revue française d’études cliniques et biologiques 12:544–558
    [Google Scholar]
  18. Stutzenberger F. J., San Clemente C. L. 1968; Production and purification of staphylococcic coagulase in a semi-defined medium. American Journal of Veterinary Research 29:1109–1116
    [Google Scholar]
  19. Tager M. 1948; Concentration, partial purification, properties and nature of staphylocoagulase. Journal of Biological Medicine 20:487–501
    [Google Scholar]
  20. Tager M. 1974; Current views on the mechanism of coagulase action in blood clotting. Annals of the New York Academy of Sciences 236:277–291
    [Google Scholar]
  21. Zolli Z., San Clemente C. L. 1963; Purification and characterization of staphylocoagulase. Journal of Bacteriology 86:527–535
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-109-2-237
Loading
/content/journal/micro/10.1099/00221287-109-2-237
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error