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High yields of staphylocoagulase from Staphylococcus aureus 104 were obtained in a simple salts medium supplemented with glycerol, casein hydrolysate and three vitamins. Conditions of oxygen-limitation (dissolved oxygen concentration ≪2%), a pH of 7·4, a temperature of 35°C and a 1 in 10 inoculum of overnight culture were required for optimal yields of staphylocoagulase.
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