Further Observations on Carbohydrate Metabolism and its Regulation in Free

Abstract

Certain enzymes of glucose catabolism in were studied and their activities in steady-state chemostat cultures were measured under various nutrient limitations. 2-Keto-3-deoxy-6-phosphogluconate aldolase was separated from 6-phosphogluconate dehydratase by affinity chromatography and the previously observed inhibition of the Entner-Doudoroff enzymes by tricarboxylic acids and ATP was attributed to chelation of Mn and Mg which activate the dehydratase. Glucose-6-phosphate dehydrogenase was unaffected by phosphopyruvate while fructose-1,6-bisphosphate aldolase was activated by Co, K or NH ions. Transketolase, transaldolase and triosephosphate isomerase were present but previous reports of 6-phosphogluconate dehydrogenase activity were shown to be artefacts. The findings confirm the major role of the Entner-Doudoroff pathway in glucose catabolism in . Pyruvate dehydrogenase, a key enzyme for carbon entry to the tricarboxylic acid cycle and to poly--hydroxybutyrate synthesis, was inhibited by acetyl-coenzyme A and NADH.

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1978-11-01
2024-03-29
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References

  1. Blevins W. T., Feary T. W., Phibbs P. V. Jr 1975; 6-Phosphogluconate dehydratase deficiency in pleiotropiccarbohydrate-negative mutant strains of Pseudomonas aeruginosa . Journal of Bacteriology 121:942–949
    [Google Scholar]
  2. Bray G. A. 1960; A simple efficient liquid scin-tillator for counting aqueous solutions in a liquid scintillation counter.. Analytical Biochemistry 1:279–285
    [Google Scholar]
  3. Bresters T. W., De Abreu R. A., De Kok A., Visser J., Veeger C. 1975; The pyruvate-dehydrogenase complex from Azotobacter vinelandii . European Journal of Biochemistry 59:335–345
    [Google Scholar]
  4. De Abreu R. A., De Kok A., Veeger C. 1977; Transformation of the 4-component pyruvate dehydrogenase complex from Azotobacter vinelandii into a 3-component complex.. FEBS Letters 82:89–92
    [Google Scholar]
  5. Erfle J. D., Sauer F. 1969; The inhibitory effects of acyl-coenzyme A esters on the pyruvate and α-oxoglutarate dehydrogenase complex.. Biochimica et biophysica acta 178:441–452
    [Google Scholar]
  6. Faik P., Kornberg H. L., McEvoy-Bowe E. 1971; Isolation and properties of Escherichia coli mutants defective in 2-keto-3-deoxy-6-phosphogluconate aldolase activity.. FEBS Letters 19:225–228
    [Google Scholar]
  7. Haaker H., Veeger C. 1976; Regulation of respiration and nitrogen fixation in different types of Azotobacter vinelandii . European Journal of Biochemistry 63:499–507
    [Google Scholar]
  8. Hansen R. G., Henning H. L. 1966; Regulation of pyruvate dehydrogenase activity in Escherichia coli k-12.. Biochimica et biophysica acta 122:355–358
    [Google Scholar]
  9. Jackson F. A., Dawes E. A. 1976; Regulation of the tricarboxylic acid cycle and poly-β-hydroxy-butyrate metabolism in Azotobacter beijerinckii grown under nitrogen or oxygen limitation.. Journal of General Microbiology 97:303–312
    [Google Scholar]
  10. Meloche H. P., Wood W. A. 1966; 2-Keto-3-deoxy-6-phosphogluconate.. Methods in Enzymology 9:51–53
    [Google Scholar]
  11. Mortenson L. E., Wilson P. W. 1954; Initial stages in the breakdown of carbohydrates by Azotobacter vinelandii . Archives of Biochemistry and Biophysics 53:425–435
    [Google Scholar]
  12. Mortenson L. E., Hamilton P. B., Wilson P. W. 1955; Dissimilation of 6-phosphogluconate by Azotobacter vinelandii . Biochimica et biophysica acta 16:238–244
    [Google Scholar]
  13. Senior P. J., Dawes E. A. 1971; Poly-β-hydroxybutyrate biosynthesis and the regulation of glucose metabolism in Azotobacter beijerinckii . Biochemical Journal 125:55–66
    [Google Scholar]
  14. Senior P. J., Dawes E. A. 1973; The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii . Biochemical Journal 134:225–238
    [Google Scholar]
  15. Senior P. J., Beech G. A., Ritchie G. A. F., & Dawes E. A. 1972; The role of oxygen limitation in the formation of poly-β-hydroxybuty-rate during batch and continuous culture of Azotobacter beijerinckii . Biochemical Journal 128:1193–1201
    [Google Scholar]
  16. Shaltiel S., Er-Al Z. 1973; Hydrophobic chromatography: use for purification of glycogen synthetase.. Proceedings of the National Academy of Sciences of the United States of America 70778–781
    [Google Scholar]
  17. Still G. G., Wang C. H. 1964; Glucose catabolism in Azotobacter vinelandii . Archives of Biochemistry and Biophysics 105:126–132
    [Google Scholar]
  18. Tunail N., Schlegel H. G. 1972; Phospho-enolpyruvate, a new inhibitor of glucose-6-phosphate dehydrogenase.. Biochemical and Bio-physical Research Communications 49:1554–1560
    [Google Scholar]
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