RT Journal Article SR Electronic(1) A1 Smith, H. WilliamsYR 1978 T1 Arsenic Resistance in Enterobacteria : its Transmission by Conjugation and by Phage JF Microbiology, VO 109 IS 1 SP 49 OP 56 DO https://doi.org/10.1099/00221287-109-1-49 PB Microbiology Society, SN 1465-2080, AB A high proportion of strains of Escherichia coli (599 of 716 strains), Shigella (15 of 18), Proteus (32 of 33) and Klebsiella pneumoniae (42 of 61), but not of Salmonella (15 of 209), were resistant to sodium arsenite; the incidence of arsenite resistance was higher in animal than in human strains of E. coli. Transmissible arsenite resistance, which was mainly plasmid-borne, was common in resistant strains of K. pneumoniae (22 of 34 tested) and Salmonella (11 of 12), but not in resistant strains of E. coli (10 of 98), Proteus (0 of 32) or Shigella (0 of 6). In three K. pneumoniae strains from which the arsenite resistance genes could not be transferred by direct methods, they were mobilized by implanting the conjugative plasmids F or I intothem. In two S. typhimurium strains with transmissible arsenite resistance, the resistance genes were located in the genome of the phage with which they were lysogenized. In several of the enterobacterial strains the plasmid-borne arsenite resistance was not associated with antibiotic resistance or any other character known to be transmissible. In many of the K. pneumoniae strains, though, it was often associated with transmissible antibiotic, mercury and tellurite resistance and especially with transmissible lactose utilization. The available evidence strongly suggested that the arsenite resistance genes were located on the Lac plasmid. The conjugative plasmids in several of the K. pneumoniae strains were temperature-sensitive. Many of the arsenite-sensitive K. pneumoniae strains grew on culture media containing mixtures of sodium arsenite and sodium nalidixate at concentrations of each which by itself would not permit their growth. Arsenite-resistant K. pneumoniae cultures did not grow under such conditions but any arsenite-sensitive mutants they contained did., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-109-1-49