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Volume 109, Issue 1

Thermosensitive Antibiotic Resistance Plasmids in Enterobacteria Free

Abstract

Of 775 conjugative plasmids found in enterobacteria mediating antibiotic resistance, 24 (3·1%) were thermosensitive (ts); they were most common in . Ts plasmids were also found in all the samples of sewage and river water examined. Over half of 73 ts plasmids from unrelated sources mediated resistance to chloramphenicol in addition to several other antibiotics. Many of them mediated resistance to mercury (53·4%), arsenite (38·4%) and tellurite (79·5%) but not to copper, cobalt and silver. Fifty-eight belonged to incompatibility group H2 and 12 belonged to the H1 group. Resistance to mercury, arsenite and tellurite was common in strains containing H2 plasmids but not in H1 plasmids. The 73 plasmids transferred at high rates at 22 and 28°C and at lower rates at 15°C; they transferred at very low rates or not at all at 37°C. They could be divided into two sets according to whether they transferred at a high or at a low rate at 33°C. Unlike the prototype plasmid, Rts 1, they were solely or mainly ts for transfer and not for replication and only one of them brought about a marked reduction in growth rate of its host organism at 42°C. None of the 73 plasmids mediated colicin or haemolysin production. Three plasmids, all from , mediated utilization of lactose, two of sucrose and raffinose and three, all belonging to group H1, of citrate. None of the plasmids increased the pathogenicity of for chicks or 12 for mice.

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© Society for General Microbiology, 1978
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1978-11-01
2023-06-06
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References

  1. Anderson E. S. 1975; The problem and implications of chloramphenicol resistance in the typhoid bacillus. Journal of Hygiene 74:289–299
     [Google Scholar]
  2. Butler T., Linh N. N., Arnold K., Pollack M. 1973; Chloramphenicol-resistant typhoid fever in Vietnam associated with R factor. Lancet ii:983
     [Google Scholar]
  3. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri . Journal of General Microbiology 72:543–552
     [Google Scholar]
  4. Cruickshank R. 1970 Medical Microbiology, 11. Cruickshank R. Edinburgh and London: F. & S. Livingstone;
     [Google Scholar]
  5. Datta N., Olarte J. 1974; R factors in strains of Salmonella typhi and Shigella dysenteriae isolated during epidemics in Mexico : classification by compatibility. Antimicrobial Agents and Chemotherapy 5:310–317
     [Google Scholar]
  6. Grabow W. O. K., Prozesky O. W., Burger J. S. 1975; Behaviour in a river and dam of coliform bacteria with transferable or non-transferable drug resistance. Water Research 9:777–782
     [Google Scholar]
  7. Hardy K. G., Meynell G. G., Dowman J. E., Spratt B. G. 1973; Two major groups of colicin factors; their evolutionary significance. Molecular and General Genetics 125:217–230
     [Google Scholar]
  8. Joly B., Cluzel R. 1975; The role of heavy metals and their derivatives in the selection of antibiotic-resistant gram-negative bacilli. Annales de Microbiologie 126B:51–61
     [Google Scholar]
  9. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63:399–406
     [Google Scholar]
  10. McHugh G. L., Moellering R. C., Hopkins C. C., Swartz M. N. 1975; Salmonella typhimurium resistance to silver nitrate, chloramphenicol and ampicillin. Lancet i:235–239
     [Google Scholar]
  11. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal power of blood. Journal of Hygiene 38:732–749
     [Google Scholar]
  12. Nakahara H., Ishikawa T., Sarai Y., Kondo L, Kozukue H., Mitsuhashi S. 1977a; Mercury resistance and R plasmids in Escherichia col isolated from clinical lesions in Japan. Antimicrobial Agents and Chemotherapy 11:999–1003
     [Google Scholar]
  13. Nakahara H., Ishikawa T., Sarai Y., Kondo I., Kozukue H. 1977b; Mercury resistance and R plasmids in clinical isolates of Klebsiella pneumoniae . Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene 238:51–58
     [Google Scholar]
  14. Rodriguez-Lemoine V., Jacob A. E., Hedges R. W., Datta N. 1975; Thermosensitive production of their transfer systems by group S plasmids. Journal of General Microbiology 86:111–114
     [Google Scholar]
  15. Sato G., Kodama H., Terakado N. 1974; Detection of an R factor showing temperaturesensitive transfer in Salmonella typhimurium isolated from calves. Antimicrobial Agents and Chemotherapy 5:541–543
     [Google Scholar]
  16. Smith H. Williams. 1973; Chloramphenicol resistance in Escherichia coli . Journal of Medical Microbiology 6:347–350
     [Google Scholar]
  17. Smith H. Williams. 1974; Thermosensitive transfer factors in chloramphenicol-resistant strains of Salmonella typhi . Lancet ii:281–282
     [Google Scholar]
  18. Smith H. Williams. 1977; Mobilization of non-conjugative tetracycline, streptomycin, spectinomycin and sulphonamide resistance determinants of Escherichia coli . Journal of General Microbiology 100:189–196
     [Google Scholar]
  19. Smith H. Williams, Parsell Z. E. 1975; Transmissible substrate-utilizing ability in enterobacteria. Journal of General Microbiology 87:129–140
     [Google Scholar]
  20. Summers A. O., Jacoby G. A. 1977; Plasmid-determined resistance to tellurium compounds. Journal of Bacteriology 129:276–281
     [Google Scholar]
  21. Terawaki Y., Takayasu H., Akiba T. 1967; Thermosensitive replication of a kanamycin resistance factor. Journal of Bacteriology 94:687–690
     [Google Scholar]
  22. Terawaki Y., Kishi H., Nakaya R. 1975; Integration of R plasmid Rts 1 to the gal region of the Escherichia coli chromosome. Journal of Bacteriology 121:857–862
     [Google Scholar]
  23. Yoshida Y., Terawaki Y., Nakaya R. 1974; Temperature sensitive R plasmid originated from Salmonella typhimurium . Biochemical and Biophysical Research Communications 59:361–369
     [Google Scholar]
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Thermosensitive Antibiotic Resistance Plasmids in Enterobacteria
Microbiology 109, 37 (1978); https://doi.org/10.1099/00221287-109-1-37
/content/journal/micro/10.1099/00221287-109-1-37
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