1887

Abstract

A temperature-sensitive mutant strain of has been isolated which failed to grow normally on minimal agar medium at 43 °C unless mannose was supplied as a sole carbon source. The mutant has been given the symbol for mannose relief. Under restrictive conditions (43 °C) the mutant produced extensive areas of swollen hyphae, called balloons, and wall preparations from cultures grown at 40 °C had approximately one-third of the mannose found in walls of control cultures. The strain produced a more thermolabile phosphomannose mutase than the wild-type strain, so it is suggested that the mutation is in the structural gene coding for this enzyme.

The strain failed to grow on minimal agar medium containing 0·1% (w/v) mannose and 0·9 % (w /v) glucose at 43 °C. This property was used to obtain revertants, one of which was a double mutant . Growth of the double mutant in media containing [C]mannose at 43 °C showed that 84 % of the label found in the wall occurred in mannose in comparison with 33% in the wild-type control. Autoradiography of cultures of the double mutant using [H]mannose showed predominant incorporation of label into the tip region of growing hyphae. A strain, isolated following haploidization of a diploid, was unable to grow on media containing glucose or mannose alone. The strain had reduced phosphomannose isomerase (EC 5.3.1.8) activity. The mutation, which is epistatic to has been located to linkage group VIII and the mutation to linkage group V. A pathway is presented for the utilization of mannose in .

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1978-11-01
2021-07-31
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