Analysis of DNA Content, Nitrogenase Activity and In Vivo Protein Synthesis of Rhizobium leguminosarum Bacteroids on Sucrose Gradients

References

1. Bisseling T., Van den Bos R. C., Van Kammen A., Van der Ploeg M., Van Duijn P., Houwers A. 1977; Cytofluorometrical determination of the DNA contents of bacteroids and corresponding broth-cultured Rhizobium bacteria. Journal of General Microbiology 101:79–84
   [Google Scholar]
2. Bisseling T., Van den Bos R. C., Van Kammen A. 1978; The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by Rhizobium leguminosarum. Biochimica et biophysica acta 539:1–11
   [Google Scholar]
3. Brill W. J. 1976; Control of nitrogenase synthesis in Azotobacter vinelandii. In Symbiotic nitrogen fixation (International Biological Programme 7)39–46 Nutman P. S. Cambridge: Cambridge University Press;
   [Google Scholar]
4. Davis L. C., Shah V. K., Brill W. J., Orme-Johnson W. H. 1972; Nitrogenase. II. Changes in the EPR signal of component I (iron-molybdenum protein) of Azotobacter vinelandii nitrogenase during repression and derepression. Biochimica et biophysica acta 256:512–523
   [Google Scholar]
5. Kennedy C., Eady R. R., Kondorosi E., Klavans Rekosh D. 1976; The molybdenum-iron protein of Klebsiella pneumoniae nitrogenase. Evidence for non-identical subunits from peptide ‘mapping’. BiochemicalJournal 155:383–389
   [Google Scholar]
6. Kijne J. W. 1975; The fine structure of pea root nodules. 2. Senescence and disintegration of the bacteroid tissue of Rhizobium leguminosarum. Physiological Plant Pathology 7:17–21
   [Google Scholar]
7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
   [Google Scholar]
8. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
   [Google Scholar]
9. Nicholas D. J. D., Deering J. V. 1976; Repression, derepression and activation of nitrogenase in Azotobacter vinelandii. Australian Journal of Biological Sciences 29:147–161
   [Google Scholar]
10. Reijnders L., Visser L., Aalbers A. M. J., Van Kammen A., Houwers A. 1975; A comparison of DNA from free living and endosymbiotic Rhizobium leguminosarum (strain pre). Biochimica et biophysica acta 414:206–216
    [Google Scholar]
11. Scott D. B., Farnden K. J. F., Robertson J. G. 1976; Ammonia assimilation in lupin nodules. Nature, London 263:703–705
    [Google Scholar]
12. Seto B., Mortenson L. E. 1974; In vivo kinetics of nitrogenase formation in Clostridium pasteurianum. Journal of Bacteriology 120:822–830
    [Google Scholar]
13. Shah V. K., Davis L. C., Brill W. J. 1972; Nitrogenase. I. Repression and derepression of the iron-molybdenum and iron proteins of nitrogenase in Azotobacter vinelandii. Biochimica et biophysica acta 256:498–511
    [Google Scholar]
14. Shah V. K., Davis L. C., Brill W. J. 1975; Nitrogenase. VI. Acetylene reduction assay: dependence of nitrogen fixation estimates on component ratio and acetylene concentration. Biochimica et biophysica acta 384:353–359
    [Google Scholar]
15. Sutton W. D., Mahoney P. 1977; Preparation and fractionation of Rhizobium bacteroids by zone sedimentation through sucrose gradients. Plant Physiology 60:800–802
    [Google Scholar]
16. Sutton W. D., Jepsen N. M., Shaw B. D. 1977; Changes in the number, viability and aminoacid incorporating activity of Rhizobium bacteroids during lupin nodule development. Plant Physiology 59:741–744
    [Google Scholar]
17. Sutton W. D., Van den Bos R. C., Bisseling T. 1978; The DNA content of Rhizobium lupini bacteroids and bacteria. Plant Science Letters 12:145–149
    [Google Scholar]
18. Thorneley R. N. F., Eady R. R., Yates M. G. 1975; Nitrogenases of Klebsiella pneumoniae and Azotobacter chroococcum. Complex formation between the component proteins. Biochimica et biophysica acta 403:269–284
    [Google Scholar]
19. Van Brussel A. A. N. 1973 The cell wall of bacteroids of Rhizobium leguminosarum Frank. Ph.D. thesis University of Leiden, The Netherlands;
    [Google Scholar]
20. Van Brussel A. A. N., Planqué K., Quispel A. 1977; The wall of Rhizobium leguminosarum in bacteroid and free-living forms. Journal of General Microbiology 101:51–56
    [Google Scholar]
21. Van Straten J., Roelofsen W. 1976; Improved method for preparing anaerobic bacteroid suspensions of Rhizobium leguminosarum for the acetylene reduction assay. Applied and Environmental Microbiology 31:859–863
    [Google Scholar]