1887

Abstract

Mutations leading to increased sensitivity to Co gamma rays and/or ultraviolet light in the slime mould have been assigned to eight loci on the basis of linkage and complementation tests. The linkages of these loci are: , linkage group I; , linkage group VI; , linkage group III; , linkage group IV; , linkage group IV; , linkage group III; , linkage group II; and , linkage group III. The mutants, which were isolated on the basis of increased sensitivity to ultraviolet light, are as resistant as their parental strains to Co gamma rays, whereas all other mutants studied are sensitive to both ultraviolet light and Co gamma rays. A new temperature-sensitive mutation, , has been assigned to linkage group I.

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1978-11-01
2021-07-29
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References

  1. Coukell M. B. 1977; Evidence against mutational ‘Hot Spots’ at aggregation loci in Dictyostelium discoideum. Molecular and General Genetics 151:269–273
    [Google Scholar]
  2. Deering R. A. 1975; Dictyostelium discoideum: a valuable eukaryotic system for repair studies. Molecular Mechanisms for Repair of DNA581–584 New York: Plenum;
    [Google Scholar]
  3. Deering R. A., Jensen D. S. 1973; Nuclear and mitochondrial DNA synthesis in gamma ray-resistant and -sensitive slime mold amoebas. Biophysical Journal 13:2780–794
    [Google Scholar]
  4. Deering R. A., Jensen D. S. 1975; Endonuclease from Dictyostelium discoideum that attacks ultraviolet-irradiated deoxyribonucleic acid. Journal of Bacteriology 121:1211–1213
    [Google Scholar]
  5. Deering R. A., Smith M. S., Thompson B. K., Adolf A. C. 1970; Gamma-ray-resistant and -sensitive strains of slime mold (Dictyostelium discoideum). Radiation Research 43:711–728
    [Google Scholar]
  6. Free S. J., Schimke R. T., Loomis W. F. 1976; The structural gene for a-mannosidase-1 in Dictyostelium discoideum. Genetics 84:159–174
    [Google Scholar]
  7. Freim J. O., Deering R. A. 1970; Ultraviolet irradiation of the vegetative cells of Dictyostelium discoideum. Journal of Bacteriology 102:36–42
    [Google Scholar]
  8. Guialis A., Deering R. A. 1976a; Repair of deoxyribonucleic acid in ultraviolet light-sensitive and -resistant Dictyostelium discoideum strains. Journal of Bacteriology 127:59–66
    [Google Scholar]
  9. Guialis A., Deering R. A. 1976b; Inhibition of UV-induced DNA strand breakage in Dictyostelium discoideum by 2,4-dinitrophenol. Photo-chemistry and Photobiology 24:331–336
    [Google Scholar]
  10. Katz E. R., Sussman M. 1972; Parasexual recombination in Dictyostelium discoideum: selection of stable diploid heterozygotes and stable haploid segregants. Proceedings of the National Academy of Science of the United States of America 69495–498
    [Google Scholar]
  11. Kessin R. H., Williams K. L., Newell P. C. 1974; Linkage analysis in Dictyostelium discoideum using temperature-sensitive growth mutants selected with bromodeoxyuridine. Journal of Bacteriology 119:776–783
    [Google Scholar]
  12. Khoury A. T., Deering R. A. 1973; Sedimentation of DNA of Dictyostelium discoideum lysed on alkaline sucrose gradients: role of single-strand breaks in gamma ray lethality of sensitive and resistant strains. Journal of Molecular Biology 79:267–284
    [Google Scholar]
  13. Loomis W. F. 1969; Temperature-sensitive mutants of Dictyostelium discoideum. Journal of Bacteriology 99:65–69
    [Google Scholar]
  14. Newell P. C., Henderson R. F., Mosses D., Ratner D. I. 1977a; Sensitivity to Bacillus subtilis: a novel system for selection of heterozygous diploids of Dictyostelium discoideum. Journal of General Microbiology 100:207–212
    [Google Scholar]
  15. Newell P. C., Ratner D. I., Wright M. D. 1977b; New techniques for cell fusion and linkage analysis of Dictyostelium discoideum. Development and Differentiation in the Cellular Slime Moulds51–61 Amsterdam: Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  16. Perkins D. D. 1962; Preservation of Neurospora stock cultures with anhydrous silica gel. Canadian Journal of Microbiology 8:591–594
    [Google Scholar]
  17. Ratner D. I., Newell P. C. 1978; Linkage analysis in Dictyostelium discoideum using multiply marked tester strains: establishment of linkage group VII and the reassessment of earlier linkage data. Journal of General Microbiology 109:
    [Google Scholar]
  18. Rothman F. G., Alexander E. T. 1975; Parasexual genetic analysis of the cellular slime mold Dictyostelium discoideum A3. Genetics 80:715–731
    [Google Scholar]
  19. Sackin M. J., Ashworth J. M. 1969; An analysis of the distribution of volumes amongst spores of the cellular slime mould Dictyostelium discoideum. Journal of General Microbiology 59:275–284
    [Google Scholar]
  20. Welker D. L., Deering R. A. 1976; Genetic analysis of radiation-sensitive mutations in the slime mould Dictyostelium discoideum. Journal of General Microbiology 97:1–10
    [Google Scholar]
  21. Welker D. L., Deering R. A. 1978a; In vivo nicking and rejoining of nuclear DNA in ultraviolet-irradiated radiation-resistant and -sensitive strains of Dictyostelium discoideum. Molecular and General Genetics
    [Google Scholar]
  22. Welker D. L., Deering R. A. 1978b; Interactions between radiation-sensitive mutations in double-mutant haploids of Dictyostelium discoideum. Moleculai and General Genetics
    [Google Scholar]
  23. Williams K. L., Newell P. C. 1976; A genetic study of aggregation in the cellular slime mould Dictyostelium discoideum using complementation analysis. Genetics 82:287–307
    [Google Scholar]
  24. Williams K. L., Kessin R. H., Newell P. C. 1974a; Genetics of growth in axenic medium of the cellular slime mould Dictyostelium discoideum. Nature, London 247:142–143
    [Google Scholar]
  25. Williams K. L., Kessin R. H., Newell P. C. 1974b; Parasexual genetics in Dictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. Journal of General Microbiology 84:59–69
    [Google Scholar]
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