Esterases of 62 and strains were analysed by horizontal slab electrophoresis in polyacrylamide-agarose gel. Five principal bands hydrolysed β-naphthyl acetate but differed in their range of activity towards other substrates and in their sensitivity to di-isofluoropropyl phosphate. Additional bands were detected in some strains of and The comparative distribution of bands showed that the four species were characterized by distinct electrophoretic patterns of their esterases. and appeared to be more closely related to one another than to In the case of and , congruence was found between biovars and esterase patterns. The numerous electrophoretypes observed within species might provide useful epidemiological markers.


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