P22 lysates of some mutants (LD mutants) of gave lower transduction frequencies than phages grown on the wild type. The lower frequency was not due to the production of reduced numbers of transducing particles, or superinfection exclusion, or decreased incidence of integration. The defect was in a host-controlled modification-restriction system. LD mutants possess The degree of expression of transducing particles varied with the phenotype selected, although some gene products, including the numbers of plaque-forming phages of P22, were not affected. However, several other Salmonella phages, some of them related to P22, exhibited high sensitivities. The LD system appeared to be identical to Colson's SA system (Colson & van Pel, 1974).


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