1887

Abstract

Synchronous cultures of the soil amoeba , prepared by a size selection procedure involving minimum metabolic perturbation, divided with a high degree of synchrony and showed a discrete S-phase of DNA synthesis. Oxygen uptake rates doubled overall during one cell cycle time of 7 to 8 h but rose to seven distinct maxima during this period. Control experiments showed that the selection procedure did not give rise to the oscillations. The maxima of respiration were more sensitive to inhibition by cyanide than were the minima of respiration; the effect of carbonyl cyanide -trifluoro-methoxyphenylhydrazone (an uncoupler of oxidative phosphorylation) was, however, greater at respiratory minima, than at the maxima. Pool levels of ATP, ADP and AMP also oscillated during the cell cycle with maximum amplitudes (peak-trough, % minimal values) of 108, 194 and 520, respectively. Adenylate charge values varied between 0·63 and 0·88. Respiratory maxima were in phase with maximum ADP levels but out of phase with maxima of ATP/ADP ratios. These results suggest that the overall changes in respiration rates during the cell cycle of are the result of respiratory control.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-108-2-197
1978-10-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/108/2/mic-108-2-197.html?itemId=/content/journal/micro/10.1099/00221287-108-2-197&mimeType=html&fmt=ahah

References

  1. Atkinson D. E., Walton G. M. 1967; Adenosine triphosphate conservation in metabolic regulation. Journal of Biological Chemistry 242:3239–3241
    [Google Scholar]
  2. Blumenthal L. K., Zahler S. A. 1962; Index for measurement of synchronization of cell populations. Science 135:724
    [Google Scholar]
  3. Chagla A. H., Griffiths A. J. 1978; Synchronous cultures of Acanthamoeba castellanii and their use in the study of encystation. Journal of General Microbiology 108:39–43
    [Google Scholar]
  4. Chance B. 1959a; Phosphorylation efficiency of the intact cell. II. Crossover phenomena in Bakers’ Yeast. Journal of Biological Chemistry 234:3036–3040
    [Google Scholar]
  5. Chance B. 1959b; Phosphorylation efficiency of the intact cell. III. Phosphorylation and de-phosphorylation in yeast cells. Journal of Biological Chemistry 234:3041–3043
    [Google Scholar]
  6. Chance B., Hollunger G. 1963; Inhibition of electron and energy transfer in mitochondria. I. Effects of amytal, thiopental, progesterone and methylene glycol. Journal of Biological Chemistry 278:418–431
    [Google Scholar]
  7. Chance B., Williams G. R. 1956; The respiratory chain and oxidative phosphorylation. Advances in Enzymology 17:65–134
    [Google Scholar]
  8. Chance B., Williams G. R., Hollunger G. 1963; Inhibition of electron and energy transfer in mitochondria. III. Spectroscopic and respiratory effects of uncoupling agents. Journal of Biological Chemistry 278:439–444
    [Google Scholar]
  9. Chapman A. G., Atkinson D. E. 1977; Adenine nucleotide concentrations and turnover rates. Their correlation with biological activity in bacteria and yeast. Advances in Microbial Physiology 15:253–306
    [Google Scholar]
  10. Chapman A. G., Fall L., Atkinson D. E. 1971; Adenosine triphosphate and synchronous mitosis in Physarum polycephalum . Journal of Bacteriology 96:330–337
    [Google Scholar]
  11. Delmer D. P. T., Brody S. 1975; Circadian rhythms in Neurospora crassa Oscillation in the level of an adenine nucleotide. Journal of Bacteriology 111:548–553
    [Google Scholar]
  12. Edwards C., Jones C. W. 1977; Respiratory properties of synchronous cultures of Alcaligenes eutrophus h16 prepared by a continuous-flow size selection method. Journal of General Microbiology 99:383–388
    [Google Scholar]
  13. Edwards S. W., Lloyd D. 1978; Properties of mitochondria isolated from cyanide-stimulated and cyanide-sensitive cultures of Acanthamoeba castellanii . Biochemical Journal 174:203–211
    [Google Scholar]
  14. Edwards C, Statham M., Lloyd D. 1975; The preparation of large-scale synchronous cultures of the trypanosomatid, Crithidia fasciculata by cell-size selection: changes in respiration and adenylate charge through the cell-cycle. Journal of General Microbiology 88:141–152
    [Google Scholar]
  15. Edwards S. W., Chagla A. H., Griffiths A. J., Lloyd D. 1977; The cytochromes of Acanthamoeba castellanii . Biochemical Journal 168:113–121
    [Google Scholar]
  16. Goodwin B. C. 1976 Analytical Physiology of Cells and Developing Organisms London and New York: Academic Press;
    [Google Scholar]
  17. Griffiths A. J., Chagla A. H. 1972; The growth and encystment of Acanthamoeba castellanii . Journal of Protozoology S19: Abstr. 130
    [Google Scholar]
  18. Hess B., Chance B. 1959; Phosphorylation efficiency of the intact cell. I. Glucose-oxygen titrations in ascites tumour cells. Journal of Biological Chemistry 234:3031–3035
    [Google Scholar]
  19. Rissane J. M., Robins E. 1958; The fluoro-metric measurement of DNA in animal tissues with special reference to the central nervous system. Journal of Biological Chemistry 233:184–188
    [Google Scholar]
  20. Lloyd D., Brookman J. S. G. 1967; An oxygen electrode reaction vessel. Biotechnology and Bioengineering 9:271–272
    [Google Scholar]
  21. Lloyd D., Phillips C. A., Statham M. 1978; Oscillations of respiration, adenine nucleotide levels and heat evolution in synchronous cultures of Tetrahymena pyriformis st prepared by continuous-flow selection. Journal of General Microbiology 106:19–26
    [Google Scholar]
  22. Poole R. K., Lloyd D., Kemp R. B. 1973; Respiratory oscillations and heat evolution in synchronously dividing cultures of the fission yeast Schizosaccharomyces pombe 972h . Journal of General Microbiology 77:209–220
    [Google Scholar]
  23. Pye K., Chance B. 1966; Sustained oscillations of reduced pyridine nucleotide in a cell-free extract of Saccharomyces carlsbergensis . Proceedings of the National Academy of Sciences of the United States of America 55888–894
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-108-2-197
Loading
/content/journal/micro/10.1099/00221287-108-2-197
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error