Bud Formation and the Inducibility of Pseudo-mycelium Outgrowth During Release from Stationary Phase in Free

Abstract

The kinetics of bud formation at 25 °C and germ tube formation at 37 °C were examined in populations of released from stationary phase by dilution into fresh nutrient medium. Evidence is presented for four separate isolates and three different defined media that: (i) cells do not deplete the media of growth-limiting nutrients when they enter stationary phase; (ii) cells accumulate as singlets when they enter stationary phase, presumably at a point early in the cell cycle; (iii) daughter cells do not separate from mother cells during the first three to four cell divisions following release from stationary phase at 25 °C; and (iv) released cells cannot be induced to form germ tubes by an increase in temperature once they have formed their first bud at 25 °C. The relationships between the lack of cell separation, the inducibility of tube formation and stationary phase are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-108-2-173
1978-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/108/2/mic-108-2-173.html?itemId=/content/journal/micro/10.1099/00221287-108-2-173&mimeType=html&fmt=ahah

References

  1. Cabib E., Bowers B. 1971; Chitin and yeast budding. Localization of chitin in yeast bud scars. Journal of Biological Chemistry 246:152–159
    [Google Scholar]
  2. Chaffin W. L., Sogin S. J. 1976; Germ tube formation from zonal rotor fractions of Candida albicans . Journal of Bacteriology 126:771–776
    [Google Scholar]
  3. Ferguson R., Soll D. R. 1977; Soluble factors and the regulation of early rate-limiting events in slime mold morphogenesis. Experimental Cell Research 106:159–165
    [Google Scholar]
  4. Hartwell L. 1974; Saccharomyces cerevisiae cell cycle. Bacteriological Reviews 38:164–198
    [Google Scholar]
  5. Land G. A., McDonald W. C., Stjernholm R. L., Friedman L. 1975; Factors affecting filamentation in Candida albicans: changes in respiratory activity of Candida albicans during filamentation. Infection and Immunity 12:119–127
    [Google Scholar]
  6. Lee K. L., Buckley H. R., Campbell C. 1975; An amino acid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans . Sabouraudia 13:148–153
    [Google Scholar]
  7. Lingappa B. T., Lingappa Y. 1967; Alkaloids as self inhibitors of fungi. Nature London: 214516–517
    [Google Scholar]
  8. Morris E. O. 1966; Aggregation of unicells: yeasts. The Fungi II63–82 Ainsworth G. C., Sussman A. New York: Academic Press;
    [Google Scholar]
  9. Pringle J.R., Mor J. 1975; Methods for monitoring the growth of yeast cultures and for dealing with the clumping problem. Methods in Cell Biology XI:131–168
    [Google Scholar]
  10. Saltarelli C. G. 1973; Growth stimulation and inhibition of Candida albicans by metabolic byproducts. Mycopathologia et mycologia applicata 51:53–63
    [Google Scholar]
  11. Sherr G. H., Weaver R. H. 1953; The dimorphism phenomenon in yeasts. Bacteriological Reviews 17:51–92
    [Google Scholar]
  12. Soll D.R., Yarger J., Mirick M. 1976; Stationary phase and the cell cycle of Dictyostelium discoideum in liquid nutrient medium. Journal of Cell Science 20:513–523
    [Google Scholar]
  13. Yarger J., Stults K., Soll D. R. 1974; Observations on the growth of Dictyostelium discoidum in axenic medium: evidence for an extracellular growth inhibitor synthesized by stationary phase cells. Journal of Cell Science 14:681–690
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-108-2-173
Loading
/content/journal/micro/10.1099/00221287-108-2-173
Loading

Data & Media loading...

Most cited Most Cited RSS feed