1887

Abstract

Over 60% of streptomycete phage, isolated from soil, were adsorbed by unsubstituted or sodium-treated kaolin, when suspended in nutrient broth, but less phage were adsorbed by sodium and calcium montmorillonite or by calcium- and aluminium-treated kaolin. The montmorillonites did, however, partially protect one of the phage from damage during experimental manipulations. Adsorption to kaolin was not influenced by pH and most of the adsorbed phage retained their infectivity. Attempts to obtain free infectious phage by desorption were unsuccessful. The pH stabilities of adsorbed and free phage differed: 50% inactivation of phage adsorbed to kaolin occurred at pH 6·1 whereas for free phage 50% inactivation occurred at pH 4·9. This suggested that ΔpH (pH–pH) was about 1·2 units, i.e. similar to previous estimates based on the activity of enzymes adsorbed to kaolin.

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/content/journal/micro/10.1099/00221287-108-1-97
1978-09-01
2024-04-18
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References

  1. Bystricky V., Stotzky G., Schiffenbauer M. 1975; Electron microscopy of T1 bacteriophage adsorbed to clay minerals: application of the critical point drying method. Canadian Journal of Microbiology 21:1278–1282
    [Google Scholar]
  2. Casida L. E., Liu W. 1974; Arthrobacter globiformis and its bacteriophage in soil. Applied Microbiology 28:951–959
    [Google Scholar]
  3. Lahav N. 1962; Adsorption of sodium bentonite particles on Bacillus subtilis. Plant and Soil 17:191–208
    [Google Scholar]
  4. Marshall K. C. 1968; Interaction between colloidal montmorillonite and cells of Rhizobium spp. with different ionogenic surfaces. Biochimica et biophysica acta 156:179–186
    [Google Scholar]
  5. Marshall K. C. 1969; Studies by microelectro-phoretic and microscopic techniques of the sorption of illite and montmorillonite to rhizobia. Journal of General Microbiology 56:301–306
    [Google Scholar]
  6. McLaren A. D. 1975; Soil as a system of humus and clay immobilized enzymes. Chemica scripta 8:97–99
    [Google Scholar]
  7. McLaren A. D., Estermann E. F. 1957; Influence of pH on the activity of chymotrypsin at a liquid solid interface. Archives of Biochemistry and Biophysics 68:157–160
    [Google Scholar]
  8. McLaren A. D., Skujins J. J. 1968; The physical environment of micro-organisms in soil. In The Ecology of Soil Bacteria3–24 Gray T. R. G., Parkinson D. Liverpool: Liverpool University Press;
    [Google Scholar]
  9. Ramirez J. R., McLaren A. D. 1966; Factors influencing phosphatase in soils. Enzymologia 31:23–38
    [Google Scholar]
  10. Ruddick S. M., Williams S. T. 1972; Studies on the ecology of actinomycetes in soil. V. Some factors influencing the dispersal and adsorption of spores in soil. Soil Biology and Biochemistry 4:93–103
    [Google Scholar]
  11. Santaro T., Stotzky G. 1968; Sorption between microorganisms and clay minerals as determined by the electrical sensing zone particle analyzer. Canadian Journal of Microbiology 14:299–307
    [Google Scholar]
  12. Skujins J., Pukite A., McLaren A. D. 1974; Adsorption and activity of chitinase on kaolinite. Soil Biology and Biochemistry 6:179–182
    [Google Scholar]
  13. Stotzky G., Bystricky V. 1969; Electron microscopic observation of surface interactions between clay minerals and microorganisms. Bacteriological ProceedingsA93
    [Google Scholar]
  14. Stotzky G., Rem L. T. 1966; Influence of clay minerals on microorganisms. I. Montmorillonite and kaolinite on bacteria. Canadian Journal of Microbiology 12:547–563
    [Google Scholar]
  15. Tan J. S. H., Reanney D. C. 1975; Interactions between bacteriophages and bacteria in soil. Soil Biology and Biochemistry 8:145–150
    [Google Scholar]
  16. Van Olphen H. 1963 An Introduction to Clay Colloid Chemistry New York: Interscience;
    [Google Scholar]
  17. Zvyagintsev D. G. 1962; Some principles of the adsorption of micro-organisms on ion exchange resins. Microbiology (English translation) 31:339–343
    [Google Scholar]
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