@article{mbs:/content/journal/micro/10.1099/00221287-108-1-33, author = "Stratford, Michael P. and Griffiths, Alan J.", title = "Variations in the Properties and Morphology of Cysts of Acanthamoeba castellanii", journal= "Microbiology", year = "1978", volume = "108", number = "1", pages = "33-37", doi = "https://doi.org/10.1099/00221287-108-1-33", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-108-1-33", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "The structure and behaviour of cysts of a single strain of Acanthamoeba castellanii produced in different cultural conditions were investigated. There appeared to be a gradation from the type of cysts with a wrinkled cyst wall, which were produced in monoxenic culture and in replacement medium, to the smooth, refractile cells, lacking a distinct wall, which were produced in a growth medium supplemented with MgCl2. All the cyst types exhibited depressed metabolic activity and only differed in the degree to which they were resistant to heating and low temperatures. The results are discussed in relation to the use of cyst structure as a criterion for classifying the various species of Acanthamoeba.", }