The structure and behaviour of cysts of a single strain of produced in different cultural conditions were investigated. There appeared to be a gradation from the type of cysts with a wrinkled cyst wall, which were produced in monoxenic culture and in replacement medium, to the smooth, refractile cells, lacking a distinct wall, which were produced in a growth medium supplemented with MgCl. All the cyst types exhibited depressed metabolic activity and only differed in the degree to which they were resistant to heating and low temperatures. The results are discussed in relation to the use of cyst structure as a criterion for classifying the various species of .


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