1887

Abstract

The P-1 incompatibility group plasmid R772 can mobilize the chromosome of strain 5006. The decreasing gradient of recombinant recovery frequencies found for markers which were increasingly distal to O min with plasmid D donors was not found with R772. Instead, it produced recombinants for all markers at frequencies of about 5 × 10 per donor. This is about 10-fold lower than the plasmid transfer frequency. Recombinants were stable and recombination was only detected over short segments of the chromosome which corresponded to about 10 min on the D plasmid map of the chromosome. All recombinants had inherited R772 and expressed all properties of the plasmid. Attempts to isolate variant plasmids with increased frequencies of recombinant formation were unsuccessful.

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/content/journal/micro/10.1099/00221287-108-1-103
1978-09-01
2022-01-24
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