The P-1 incompatibility group plasmid R772 can mobilize the chromosome of strain 5006. The decreasing gradient of recombinant recovery frequencies found for markers which were increasingly distal to O min with plasmid D donors was not found with R772. Instead, it produced recombinants for all markers at frequencies of about 5 x 10 per donor. This is about 10-fold lower than the plasmid transfer frequency. Recombinants were stable and recombination was only detected over short segments of the chromosome which corresponded to about 10 min on the D plasmid map of the chromosome. All recombinants had inherited R772 and expressed all properties of the plasmid. Attempts to isolate variant plasmids with increased frequencies of recombinant formation were unsuccessful.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error