%0 Journal Article %A LINDBERG, ALF A. %A HELLERQVIST, CARL G. %A BAGDIAN-MOTTA, GENEVIÈVE %A MÄKELÄ, P. HELENA %T Lipopolysaccharide Modification Accompanying Antigenic Conversion by Phage P27 %D 1978 %J Microbiology, %V 107 %N 2 %P 279-287 %@ 1465-2080 %R https://doi.org/10.1099/00221287-107-2-279 %I Microbiology Society, %X A structural study by methylation analysis was made of the lipopolysaccharides from Salmonella bredeney and strains of Salmonella typhimurium which had been lysogenized with phage P27 and thereby converted to have the new O-antigen factor 27. The results were compared with previous studies on the non-converted, 27-, parental strains. The change from 27- to 27+ was accompanied by a change in the linkage between the repeating units of the O side-chain from galactose-(1→2)-mannose to galactose-(1→6)-mannose. In S. bredeney (P27), where the expression of antigen 27 is stable, 98 % of the linkages were changed, whereas only 31 to 45 % of the linkages were affected in two strains of S. typhimurium (P27). As 71 to 85 % of the cells in the same batches of S. typhimurium bacteria were 27+ it seems probable that in these strains many bacteria contained both types of linkages in their lipopolysaccharide. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-107-2-279