1887

Abstract

A structural study by methylation analysis was made of the lipopolysaccharides from and strains of which had been lysogenized with phage P27 and thereby converted to have the new O-antigen factor 27. The results were compared with previous studies on the non-converted, 27, parental strains. The change from 27 to 27 was accompanied by a change in the linkage between the repeating units of the O side-chain from galactose-(1→2)-mannose to galactose-(1→6)-mannose. In (P27), where the expression of antigen 27 is stable, 98 % of the linkages were changed, whereas only 31 to 45 % of the linkages were affected in two strains of (P27). As 71 to 85 % of the cells in the same batches of bacteria were 27 it seems probable that in these strains many bacteria contained both types of linkages in their lipopolysaccharide.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-107-2-279
1978-08-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/107/2/mic-107-2-279.html?itemId=/content/journal/micro/10.1099/00221287-107-2-279&mimeType=html&fmt=ahah

References

  1. Bagdian G., Mäkelä P. H. 1971; Antigenic conversion by phage P27.1. Mapping of the prophage attachment site on the Salmonella chromosome. Virology 43:403–411
    [Google Scholar]
  2. Bagdian G., Lüderitz O., Staub A. M. 1966; Immunochemical studies on Salmonella XI. Chemical modification correlated with conversion of group B Salmonella by bacteriophage 27. Annals of the New York Academy of Sciences 133:405–424
    [Google Scholar]
  3. Björndal H., Lindberg B., Svensson S. 1967a; Gas-liquid chromatography of partially methylated alditols as their acetates. Acta chemica scandinavica 21:1801–1804
    [Google Scholar]
  4. Björndal H., Lindberg B., Svensson S. 1967b; Mass spectrometry of partially methylated alditol acetates. Carbohydrate Research 5:433–440
    [Google Scholar]
  5. DeBelder A. N., Norrman B. 1968; The distribution of substituents in partially acetylated dextran. Carbohydrate Research 8:1–6
    [Google Scholar]
  6. Dröge W., Ruschmann E., Lüderitz O., Westphal O. 1968; Biochemical studies on lipopolysaccharides of Salmonella R mutants. 4. Phosphate groups linked to heptose units and their absence in some R lipopolysaccharides. European Journal of Biochemistry 4:134–138
    [Google Scholar]
  7. Golovkina L. S., Chizov O. S., Wulfson N. S. 1966; Mass spectrometer investigation of carbohydrates. Communication 9. Acetates of polyols. Bulletin of the Academy of Sciences of the USSR. Division of Chemical Science 11:1853–1863
    [Google Scholar]
  8. Hellerqvist C. G., Lindberg A. A. 1971; Structural studies of the commoncore poly-saccharide of the cell-wall lipopolysaccharide from Salmonella typhimurium . Carbohydrate Research 16:39–48
    [Google Scholar]
  9. Hellerqvist C. G., Lindberg B., Svensson S., Holme T., Lindberg A. A. 1968; Structural studies on the O-specific side-chains of the cell-wall lipopolysaccharide of Salmonella typhimurium 395ms . Carbohydrate Research 8:43–55
    [Google Scholar]
  10. Hellerqvist C. G., Larm O., Lindberg B., Holme T., Lindberg A. A. 1969; Structural studies on the O-specific side-chains of the cell-wall lipopolysaccharide from Salmonella bredeney . Acta chemica scandinavica 23:2217–2222
    [Google Scholar]
  11. Hellerqvist C. G., Larm O., Lindberg B., Lindberg A. A. 1971a; Structure of an oligo-saccharide obtained on degradation of the lipopolysaccharide from Salmonella typhimurium lt2. Acta chemica scandinavica 25:744–745
    [Google Scholar]
  12. Hellerqvist C. G., Lindberg B., Samuelsson K., Lindberg A. A. 1971b; Structural studies on the O specific side-chains of the cell-wall lipopolysaccharide from Salmonella paratyphi A var. durazzo . Acta chemica scandinavica 25:955–961
    [Google Scholar]
  13. Hellerqvist C. G., Hoffman J., Lindberg B., Pilotti Å., Lindberg A. A. 1971c; Anomeric nature of the d-mannose residues in the Salmonella typhi and S. Strasbourg lipopolysaccharides. Acta chemica scandinavica 25:1512–1513
    [Google Scholar]
  14. Kauffman F. 1966 Die Bakteriologie der Salmonella Species Copenhagen: Munksgaard;
    [Google Scholar]
  15. Kauffmann F., Rhode R. 1961; NeueBefunden beim O-Formenwechsel der Salmonella Species. Acta pathologica et microbiologica scandinavica 52:211–216
    [Google Scholar]
  16. Kotelko K., Staub A. M., Tinelli R. 1961; Étude immunochimique des Salmonella VIII. Rôle des groupements O-acétyls dans la spécificité du facteur O:5. Annales de l’Institut Pasteur 100:618–637
    [Google Scholar]
  17. LeMinor L. 1962; Conversion par lysogénisation de quelques sérotypes de Salmonella des groupes A, B et D normalement dépourves du facteur 0:27 en cultures 27 positives. Annales de l’Institut Pasteur 103:684–706
    [Google Scholar]
  18. LeMinor L., LeMinor S., Nicolle P. 1961; Conversion de cultures de Salmonella schwarzen-grund et Salmonella bredeney en cultures 27 positives par la lysogénisation. Annales de l’Institut Pasteur 101:571–589
    [Google Scholar]
  19. Lindberg A. A., Hellerqvist C. G. 1971; Bacteriophage attachment sites, serological specificity, and chemical composition of the lipopolysaccharides of semirough and rough mutants of Salmonella typhimurium . Journal of Bacteriology 105:57–64
    [Google Scholar]
  20. Lindberg A. A., Holme T. 1969; Influence of O side-chains on the attachment of the Felix O-1 bacteriophage to Salmonella bacteria. Journal of Bacteriology 99:513–519
    [Google Scholar]
  21. Lindberg A. A., Holme T. 1972; Evaluation of some extraction methods for the preparation of bacterial lipopolysaccharides for structural analysis. Acta pathologica et microbiologica scandinavica b 80:751–759
    [Google Scholar]
  22. Naide Y., Nikaido H., Mäkelä P. H., Wilkinson R. G., Stocker B. A. D. 1965; Semi-rough strains of Salmonella . Proceedings of the National Academy of Sciences of the United States of America 53147–153
    [Google Scholar]
  23. Nikaido H. 1965; Biosynthesis of cell-wall polysaccharide in mutant strains of Salmonella III. Transfer of l-rhamnose and d-galactose. Biochemistry 4:1550–1561
    [Google Scholar]
  24. Nikaido H., Nikaido K. 1965; Biosynthesis of cell-wall polysaccharide in mutant strains of Salmonella IV. Synthesis of S-specific side-chains. Biochemical and Biophysical Research Communications 19:322–327
    [Google Scholar]
  25. Nurminen M., Hellerqvist C. G., Valtonen V.V., Mäkelä P. H. 1971; The smooth lipopolysaccharide character of 1, 4, 5, 12 trans-ductants formed as hybrids between groups B and D of Salmonella . xEuropean Journal of Biochemistry 22:500–505
    [Google Scholar]
  26. Osborn M. J. 1969; Structure and biosynthesis of the bacterial cell-wall. Annual Review of Biochemistry 38:501–538
    [Google Scholar]
  27. Sawardeker J. S., Slonecker J. H., Jeanes A. 1965; Quantitative determination of mono-saccharides as their alditol acetates by gas liquid chromatography. Analytical Chemistry 37:1602–1604
    [Google Scholar]
  28. Spicer C. C., Datta N. 1959; Reversion of transduced antigenic characters in Salmonella typhimurium . Journal of General Microbiology 20:136–143
    [Google Scholar]
  29. Staub A. M., Bagdian G. 1966; Études immunochimiques sur les Salmonella XII. Analyse immunologique des facteurs 27A, 27B et 27D. Annales de l’Institut Pasteur 110:849–860
    [Google Scholar]
  30. Stocker B. A. D., Staub A. M., Tinelli R., Kopacka B. 1960; Étude immunochimique sur les Salmonella VI. Étude de l’antigene 1 present sur deux Salmonella des groupes B et E4. Annales de l’Institut Pasteur 98:505–523
    [Google Scholar]
  31. Tinelli R., Staub A. M. 1960; Analyse de l’antigène O12 du tableau de Kauffmann-White. Bulletin de la Sociétéde chimie biologique 42:583–599
    [Google Scholar]
  32. Westphal O., Lüderitz O., Bister F. 1952; Über die Extraktion von Bakterien mit Phenol Wasser. Zeitschrift für Naturforschung 7b:148–155
    [Google Scholar]
  33. Whistler R. L., Wolfrom M. L. 1962 Methods in Carbohydrate Chemistry. I. Analysis and Preparation of Sugars New York and London: Academic Press;
    [Google Scholar]
  34. Zinder N. D., Lederberg J. 1952; Genetic exchange in Salmonella . Journal of Bacteriology 64:679–699
    [Google Scholar]
/content/journal/micro/10.1099/00221287-107-2-279
Loading
/content/journal/micro/10.1099/00221287-107-2-279
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error