1887

Abstract

Observations by phase contrast, fluorescence and electron microscopy showed that epimastigotes of () (grown ) were phagocytosed posterior end first by mouse peritoneal macrophages . Many were subsequently digested as a result of phagosome-lysosome fusion but others survived by apparently inhibiting this fusion and/or escaping from the phagosome into the host cell's cytoplasm. These survivors replicated as amastigotes. Long trypomastigotes, separated from populations grown by passage down a column of glass beads (with or without prior exposure to guinea-pig serum), were phagocytosed by either pole and all were subsequently digested.

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1978-08-01
2021-05-19
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References

  1. Akiyama H. J., Haight R. D. 1971; Interaction of Leishmania donovani and hamster peritoneal macrophages. A phase contrast microscopical study. American Journal of Tropical Medicine and Hygiene 20:539–545
    [Google Scholar]
  2. Alexander J., Vickerman K. 1975; Fusion of host cell secondary lysosomes with the parasit-ophorous vacuoles of Leishmania mexicana-infected macrophages. Journal of Protozoology 22:502–508
    [Google Scholar]
  3. Baker J. R., Liston A. J. 1978; Trypanosoma (Schizotrypanum) dionisii: effect of various agents on attachment and entry to macrophages in vitro and on morphogenesis. Journal of General Microbiology 104:79–89
    [Google Scholar]
  4. Baker J. R., Green S. M., Chaloner L. A., Gaborak M. 1972; Trypanosoma (Schizotrypanum) dionisii of Pipistrellus pipistrellus (Chiroptera) : intra- and extracellular development in vitro . Parasitology 65:251–263
    [Google Scholar]
  5. Baker J. R., Liston A. J., Selden L. F. 1976; Trypomastigote dimorphism and satellite deoxyribonucleic acid in a clone of Trypanosoma (Schizotrypanum) dionisii . Journal of General Microbiology 97:113–115
    [Google Scholar]
  6. Baker J. R., Liston A. J., Selden L. F. 1977; Early interaction between host-cell and parasite in Schizotrypanum species. Abstracts of Papers Read at the Fifth International Congress on Protozoology461 Hutner S. H. New York:
    [Google Scholar]
  7. Chang K.-P., Dwyer D. M. 1976; Multiplication of a human parasite (Leishmania donovani) in phagolysosomes of human macrophages in vitro . Science 193:678–680
    [Google Scholar]
  8. Chang K.-P., Dwyer D. M. 1978; Leishmania donovani hamster macrophage interactions in vitro : cell entry, intracellular survival, and multiplication of amastigotes. Journal of Experimental Medicine 147:515–530
    [Google Scholar]
  9. D’Arcy Hart P., Young M. R. 1975; Interference with normal phagosome-lysosome fusion in macrophages, using ingested yeast cells and suramin. Nature London: 25647–49
    [Google Scholar]
  10. Hirsch J. G., Fedorko M. E. 1968; Ultra-structure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and postfixation in uranyl acetate. Journal of Cell Biology 38:615–628
    [Google Scholar]
  11. Jones T. C., Hirsch J. G. 1972; The interaction between Toxoplasma gondii and mammalian cells. II. The absence of lysosomal fusion with phagocytic vacuoles containing living parasites. Journal of Experimental Medicine 136:1173–1194
    [Google Scholar]
  12. Khavkin Th. N., Freidlin I. S. 1977; A fluorescence phase contrast study of the interaction between Toxoplasma gondii and lysosomes in living cells. Zeitschrift für Parasitenkunde 52:19–21
    [Google Scholar]
  13. Klein R. A., Law D. T. 1976; Heated microscope stage with precise temperature control. Laboratory Practice 25:160–161
    [Google Scholar]
  14. Kress Y., Bloom B. R., Wittner M., Rowen A., Tanowitz H. 1975; Resistance of Trypanosoma cruzi to killing by macrophages. Nature London: 257394–396
    [Google Scholar]
  15. Kress Y., Tanowitz H., Bloom B. R., Wittner M. 1977; Trypanosoma cruzi: infection of normal and activated mouse macrophages. Experimental Parasitology 41:385–396
    [Google Scholar]
  16. Lewis D. H., Peters W. 1977; The resistance of intracellular Leishmania parasites to digestion by lysosomal enzymes. Annals of Tropical Medicine and Parasitology 71:295–312
    [Google Scholar]
  17. Liston A. J. 1975; Trypanosoma (Schizotrypanum) dionisii in macrophage cultures. 1. Preliminary observations on entry into cells. Parasitology 71:xviii
    [Google Scholar]
  18. Miller H. C., Twohy D. W. 1967; Infection of macrophages in culture by leptomonads of Leishmania donovani . Journal of Protozoology 14:781–789
    [Google Scholar]
  19. Milder R., Kjloetzel J., Deane M. P. 1976; Interaction of peritoneal macrophages and Trypanosoma cruzi . Revista do Instituto de medicina tropical de São Paulo 18:139
    [Google Scholar]
  20. Milder R., Kloetzel J., Deane M. P. 1977; Observations on the interaction of peritoneal macrophages with Trypanosoma cruzi II. Intracellular fate of bloodstream forms. Revista do Instituto de medicina tropical de São Paulo 19:313–322
    [Google Scholar]
  21. Nogueira N., Cohn Z. 1976; Trypanosoma cruzi: mechanism of entry and intracellular fate in mammalian cells. Journal of Experimental Medicine 143:1402–1420
    [Google Scholar]
  22. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17:206–212
    [Google Scholar]
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