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Heat-injured Streptococcus faecalis recovered tellurite resistance in 4 to 5 h when incubated at 33 °C in a non-growth medium containing KH2PO4/K2HPO4 buffer pH 7·1, glucose and casein hydrolysate. Only 10 % of the damaged population recovered in the absence of K+, or glucose, or casein hydrolysate, or in the presence of valinomycin in medium containing 1 mm-K+. The K+ content of cells increased two- to threefold on heating and a further three-to sixfold during the first 30 min of subsequent incubation. The Na+ content decreased simultaneously but Mg2+ concentrations were not affected. Recovery was not affected by adding 1 mm-EDTA or 2 mm-Mg2+ to the medium but the addition of 1 mm-EDTA and 2 mm-Mg2+ together reduced recovery to < 20 %. This effect was reversed by adding 2 mm-Ca2+.
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