1887

Abstract

Heat-injured recovered tellurite resistance in 4 to 5 h when incubated at 33 °C in a non-growth medium containing KHPO/KHPO buffer pH 7·1, glucose and casein hydrolysate. Only 10 % of the damaged population recovered in the absence of K, or glucose, or casein hydrolysate, or in the presence of valinomycin in medium containing 1 m-K. The K content of cells increased two- to threefold on heating and a further three-to sixfold during the first 30 min of subsequent incubation. The Na content decreased simultaneously but Mg concentrations were not affected. Recovery was not affected by adding 1 m-EDTA or 2 m-Mg to the medium but the addition of 1 m-EDTA and 2 m-Mg together reduced recovery to < 20 %. This effect was reversed by adding 2 m-Ca.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-107-2-217
1978-08-01
2022-10-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/107/2/mic-107-2-217.html?itemId=/content/journal/micro/10.1099/00221287-107-2-217&mimeType=html&fmt=ahah

References

  1. Clark C. W., Witter L. D., Ordal Z. J. 1968; Thermal injury and recovery of Streptococcus faecalis . Applied Microbiology 16:1764–1769
    [Google Scholar]
  2. Hamilton W. A. 1975; Energy coupling in microbial transport. Advances in Microbial Physiology 12:1–53
    [Google Scholar]
  3. Harold F. M., Baarda J. R. 1967; Gramicidin, valinomycin and cation permeability of Streptococcus faecalis . Journal of Bacteriology 94:53–60
    [Google Scholar]
  4. Harold F. M., Baarda J. R. 1968; Effects of nigericin and monactin on cation permeability of Streptococcus faecalis and metabolic capacities of potassium depleted cells. Journal of Bacteriology 95:816–823
    [Google Scholar]
  5. Harold F. M., Baarda J. R. 1969; Inhibition of membrane-bound adenosine triphosphatase and of cation transport in Streptococcus faecalis by N/N′-dicyclohexylcarbodiimide. Journal of Biological Chemistry 244:2261–2268
    [Google Scholar]
  6. Harold F. M., Papineau D. 1972; Cation transport and electrogenesis by Streptococcus faecalis II. Proton and sodium extrusion. Journal of Membrane Biology 8:45–62
    [Google Scholar]
  7. Harold F. M., Baarda J. R., Pavlasova E. 1970a; Extrusion of sodium and hydrogen ions as the primary process in potassium ion accumulation by Streptococcus faecalis . Journal of Bacteriology 101:152–159
    [Google Scholar]
  8. Harold F. M., Pavlasova E., Baarda J. R. 1970b; A transmembrane pH gradient in Streptococcus faecalis Origin and dissipation by proton conductors and N N′-dicyclohexylcarbodiimide. Biochimica et biophysica acta 196:235–244
    [Google Scholar]
  9. Hughes A., Hurst A. 1976; Magnesium requirement of Staphylococcus for repair from sub-lethal heat injury. Canadian Journal of Microbiology 22:1202–1205
    [Google Scholar]
  10. Hurst A., Hughes A., Beare-Rogers J. L., Collins-Thompson D. L. 1973; Physiological studies on the recovery of salt tolerance by Staphylococcus aureus after sub-lethal heating. Journal of Bacteriology 116:901–907
    [Google Scholar]
  11. Hurst A., Hughes A., Duckworth M., Baddiley J. 1975; Loss of d-alanine during sub-lethal heating of Staphylococcus aureus s6 and magnesium binding during repair. Journal of General Microbiology 89:277–284
    [Google Scholar]
  12. Iandolo J. J., Ordal Z. J. 1966; Repair of thermal injury of Staphylococcus aureus . Journal of Bacteriology 91:134–142
    [Google Scholar]
  13. Lubin M., Ennis H. L. 1964; On the role of intracellular potassium in protein synthesis. Biochimica et biophysica acta 80:614–631
    [Google Scholar]
  14. Martell A. E., Calvin M. 1953 Chemistry of the Metal Chelate Compounds488 New York: Prentice-Hall;
    [Google Scholar]
  15. Payne J., Morley J. S. 1976; Recovery of tellurite resistance by heat-injured Streptococcus faecalis . Journal of General Microbiology 94:421–424
    [Google Scholar]
  16. Simoni R. D., Postma P. W. 1975; The energetics of bacterial active transport. Annual Review of Biochemistry 44:523–554
    [Google Scholar]
  17. Tomlins R. I., Ordal Z. J. 1976; Thermal injury and inactivation in vegetative bacteria. Inhibition and Inactivation of Vegetative Microbes153–190 Skinner F. A., Hugo W. B. London and New York: Academic Press;
    [Google Scholar]
  18. Zarlengo M. H., Schultz S. G. 1966; Cation transport and metabolism in Streptococcus faecalis . Biochimica et biophysica acta 126:308–320
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-107-2-217
Loading
/content/journal/micro/10.1099/00221287-107-2-217
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error