1887

Abstract

Chloramphenicol-resistant were obtained by infecting myxococci with specialized transducing phage P1CM. The drug-resistant myxococci were phenotypically unstable. They contained more than one type of plasmid; these plasmids were not found in the parent strain. Chloramphenicol-resistant were obtained by transformation with either a fraction of myxococcal DNA containing the plasmids or with P1CM prophage DNA. These transformants contained plasmids. transformed by DNA from the myxococci contained both P1CM and myxococcal genes. Individual transformant clones differed in the genetic make-up of their plasmids. Among the myxococcal genes expressed in these plasmid-harbouring strains were a capacity for self-transmissibility and a pattern of phage sensitivity characteristic of R factor incompatibility group W. transformed with P1CM prophage contained incomplete P1CM genomes; none of the chloramphenicol-resistant transformants produced P1CM phage particles. The significance of these findings for an understanding of mechanisms for the generation of R factors is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-107-1-73
1978-07-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/107/1/mic-107-1-73.html?itemId=/content/journal/micro/10.1099/00221287-107-1-73&mimeType=html&fmt=ahah

References

  1. Baker F. J. 1967 Handbook of Bacteriological Technique259 London: Butterworths;
    [Google Scholar]
  2. Berns K. I., Thomas C. A. Jr 1965; Isolation of high molecular weight DNA from Hemophilus influenzae. Journal of Molecular Biology 11:476–490
    [Google Scholar]
  3. Bradley D. E. 1976; Adsorption of the R-specific bacteriophage PR4 to pili determined by a drug resistance plasmid of the W compatibility group. Journal of General Microbiology 95:181–185
    [Google Scholar]
  4. Bradley D. E., Cohen D. R. 1977; Adsorption of lipid-containing bacteriophages PR4 and PRD1 to pili determined by a P-1 incompatibility group plasmid. Journal of General Microbiology 98:619–623
    [Google Scholar]
  5. Brockman E. R. 1973; Isolation of myxobacteria from marine habitats in the U.S. Virgin Islands. In Estuarine Microbial Ecology45–51 Stevenson L. H., Colwell R. R. Columbia, South Carolina: University of South Carolina Press;
    [Google Scholar]
  6. Burchard R. P., Parish J. H. 1975; Chlor-amphenicol resistance in Myxococcus xanthus. Antimicrobial Agents and Chemotherapy 7:233–238
    [Google Scholar]
  7. Clewell D. B., Helinski D. R. 1969; Super-coiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proceedings of the National Academy of Sciences of the United States of America 621159–1166
    [Google Scholar]
  8. Crosa J. H., Brenner J., Falkow S. 1976; Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. Journal of Bacteriology 115:904–911
    [Google Scholar]
  9. Currier T. C., Nester E. W. 1976; Isolation of covalently closed circular DNA of high molecular weight from bacteria. Analytical Biochemistry 76:431–441
    [Google Scholar]
  10. Datta N., Hedges R. W. 1972; Host ranges of R factors. Journal of General Microbiology 70:453–460
    [Google Scholar]
  11. Davis R. W., Simon M., Davison N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In Methods of Enzymology XXI:413–428
    [Google Scholar]
  12. Dessev G. N., Grancharov K. 1973; Precipitation of RNA, DNA and nucleoprotein particles from very dilute solutions. Analytical Biochemistry 53:269–271
    [Google Scholar]
  13. Flamm W. G., Birnstiel M. L., Walker P. M. B. 1972; Isopycnic centrifugation of DNA: methods and applications. In Subcellular Components: Preparation and Fractionation279–310 Birnie G. D. London and Baltimore: Butterworths and University Park Press;
    [Google Scholar]
  14. Garen A., Levinthal C. 1960; A fine structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochimica et biophysica acta 38:470–483
    [Google Scholar]
  15. Ikeda H., Tomizawa J. 1968; Prophage P1, an extrachromosomal replication unit. Cold Spring Harbor Symposia on Quantitative Biology 33:791–798
    [Google Scholar]
  16. Kaiser D., Dworkin M. 1975; Gene transfer to a myxobacterium by Escherichia coli phage P1. Science 187:653–655
    [Google Scholar]
  17. Khairul Bashar S. A. M., Parish J. H., Brown M. 1971; Biosynthesis in vitro of trypto-phanase by polyribosomes from induced cultures of Escherichia coli. Biochemical Journal 123:355–365
    [Google Scholar]
  18. Mandel M., Higa A. 1970; Calcium-dependent bacteriophage DNA infection. Journal of Molecular Biology 53:159–162
    [Google Scholar]
  19. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  20. Novick R. P., Clowes R. C., Cohen S. N., Curtiss R. III, Datta N., Falkow S. 1976; Uniform nomenclature for bacterial plasmids: a proposal. Bacteriological Reviews 40:168–189
    [Google Scholar]
  21. Pakroppa W., Goebel W., Müller W. 1975; Isolation of closed circular duplex DNA by chromatography on hydroxyapatite in the presence of ethidium bromide. Analytical Biochemistry 67:372–383
    [Google Scholar]
  22. Parish J. H. 1975; Transfer of drug resistance to Myxococcus from bacteria carrying drug-resistance factors. Journal of General Microbiology 87:198–210
    [Google Scholar]
  23. Parish J. H., Wedgwood K. R., Herries D. G. 1976; Morphogenesis in Myxococcus xanthus and Myxococcus virescens (Myxobacterales). Archives of Microbiology 107:343–351
    [Google Scholar]
  24. Šatava J., Zadraižil S., Šormová Z. 1973; Isolation of transforming DNA from Bacillus subtilis by gel filtration on Sepharose 4B. Collection of Czech Chemical Communications 38:2167–2173
    [Google Scholar]
  25. Scott J. R., Kropf M. M. 1977; Location of new clear plaque genes on the P1 map. Virology 82:362–368
    [Google Scholar]
  26. Watanabe T., Furuse C., Sakaizumi S. 1968; Transduction of various R factors by phage P1 in Escherichia coli and by phage P22 in Salmonella typhimurium. Journal of Bacteriology 96:1791–1795
    [Google Scholar]
  27. Wireman J. W., Dworkin M. 1977; Develop-mentally induced autolysis during fruiting body formation by Myxococcus xanthus. Journal of Bacteriology 129:796–802
    [Google Scholar]
  28. Yun T., Vapnek D. 1977; Electron microscopic analysis of bacteriophages P1, P1CM and P7: determination of genome sizes, sequence homology and location of antibiotic resistance determinants. Virology 77:376–385
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-107-1-73
Loading
/content/journal/micro/10.1099/00221287-107-1-73
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error