1887

Abstract

Evidence is presented that in the true slime mould the ability of the amoebal strain d- to grow in axenic medium is determined by mutations in two genes and and that the axenic growth of 4- amoebae is also under genetic control. Mutant amoebal strains are also able to grow on bacterial lawns and the ability to grow in axenic media persists during prolonged culture on bacteria. However, some of the mutant strains grow less well on bacteria than strains of similar genetic background which are unable to grow in axenic media. d- has the same nuclear DNA content (0.59 pg per nucleus) as d, the strain from which it was derived. Amoebae able to grow in axenic media were also derived from strains 27 and 858 which carry mutations for temperature sensitivity and leucine requirement expressed in the plasmodial phase. Tests in axenic media showed that these mutations were expressed in the amoebal phase. The elucidation of the genetic basis of axenic growth will allow the construction of a range of amoebal strains able to grow in axenic media and this will greatly facilitate the isolation and analysis of mutants in this organism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-106-2-297
1978-06-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/106/2/mic-106-2-297.html?itemId=/content/journal/micro/10.1099/00221287-106-2-297&mimeType=html&fmt=ahah

References

  1. Adler P. N., Holt C. E. 1974; Changes in properties of Physarum polycephalum amoebae during extended culture. Journal of Bacteriology 120:532–533
    [Google Scholar]
  2. Anderson R. W., Dee J. 1977; Isolation and analysis of amoebal-plasmodial transition mutants in the myxomycete Physarum polycephalum. Genetical Research 29:21–34
    [Google Scholar]
  3. Cooke D. J., Dee J. 1974; Plasmodium formation without change in nuclear DNA content in Physarum polycephalum. Genetical Research 23:307–317
    [Google Scholar]
  4. Cooke D. J., Dee J. 1975; Methods for the isolation and analysis of plasmodial mutants in Physarum polycephalum. Genetical Research 24:175–187
    [Google Scholar]
  5. Dee J. 1966; Multiple alleles and other factors affecting plasmodium formation in the true slime mould Physarum polycephalum Schw. Journal of Protozoology 13:610–616
    [Google Scholar]
  6. Dee J., Poulter R. T. M. 1970; A gene conferring actidione resistance and abnormal morphology on Physarum polycephalum plasmodia. Genetical Research 15:35–41
    [Google Scholar]
  7. Dee J., Wheals A. E., Holt C. E. 1973; Inheritance of plasmodial valine requirement in Physarum polycephalum. Genetical Research 21:87–101
    [Google Scholar]
  8. Gingold E. C., Grant W. D., Wheals A. E., Wren M. 1976; Temperature sensitive mutants of the slime mould Physarum polycephalum. II. Mutants of the plasmodial phase. Molecular and General Genetics 149:115–119
    [Google Scholar]
  9. Goodman E. M. 1972; Axenic culture of myxa-moebae of the myxomycete Physarum poly-cepkalum. Journal of Bacteriology 111:242–247
    [Google Scholar]
  10. Haugli F. B. 1971; Mutagenesis, selection and genetic analysis in Physarum polycephalum. Ph.D. thesis University of Wisconsin; U.S.A.:
    [Google Scholar]
  11. Henney H. R., Henney M. R. 1968; Nutritional requirements for the growth in pure culture of the myxomycete Physarum rigidum and related species. Journal of General Microbiology 53:333–339
    [Google Scholar]
  12. McCullough C. H. R., Cooke D. J., Foxon J. L., Sudbery P. E., Grant W. D. 1973; Nuclear DNA content and senescence in Physarum polycephalum. Nature New Biology 245:263–265
    [Google Scholar]
  13. McCullough C. H. R., Dee J. 1976; Defined and semi-defined media for the growth of amoebae of Physarum polycephalum. Journal of General Microbiology 95:151–158
    [Google Scholar]
  14. Mirsky A. E., Ris H. 1951; The desoxyribonucleic acid content of animal cells and its evolutionary significance. Journal of General Physiology 34:451–462
    [Google Scholar]
  15. Mohberg J., Rusch H. P. 1971; Isolation and DNA content of nuclei of Physarum polycephalum. Experimental Cell Research 66:305–316
    [Google Scholar]
  16. Mohberg J. 1977; Nuclear DNA content and chromosome numbers throughout the life cycle of the Colonia strain of the myxomycete Physarum polycephalum. Journal of Cell Science 24:95–108
    [Google Scholar]
  17. Poulter R. T. M., Dee J. 1968; Segregation of factors controlling fusion between plasmodia of the slime mould Physarum polycephalum. Genetical Research 12:71–79
    [Google Scholar]
  18. Wheals A. E. 1970; A homothallic strain of the myxomycete Physarum polycephalum. Genetics 66:623–633
    [Google Scholar]
  19. Williams K. L., Kessin R. H., Newell P. C. 1974; Parasexual genetics in Dictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. Journal of General Microbiology 84:59–69
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-106-2-297
Loading
/content/journal/micro/10.1099/00221287-106-2-297
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error