1887

Abstract

A higher activity of glutamine synthetase (EC 6.3.1.2) was found in when NH was limiting as nitrogen source than when glutamate was limiting. When glutamate, glutamine or NH were in excess, a lower activity was found. Immunological titration and sucrose gradient sedimentation of the enzyme established that under all these conditions enzyme activity corresponded to enzyme concentration and that the octamer was the predominant oligomeric form. When was shifted from nitrogen-limiting substrates to excess product as nitrogen source, the concentration of glutamine synthetase was adjusted with kinetics that closely followed dilution by growth. When grown on limiting amounts of glutamate, a lower oligomer was present in addition to the octameric form of the enzyme. When the culture was shifted to excess NH , glutamine accumulated at a high rate; nevertheless, there was only a slow decrease in enzyme activity and no modification of the oligomeric pattern.

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1978-06-01
2021-07-28
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References

  1. Espin G, Mora J. 1978; Effect of the deprivation of amino acids on conidia of Neurospora crassa. Journal of General Microbiology 104:233–240
    [Google Scholar]
  2. Ferguson A. R., Sims A. P. 1974a; The regulation of glutamine metabolism in Candida utilis: the role of glutamine in the control of glutamine synthetase. Journal of General Microbiology 80:159–171
    [Google Scholar]
  3. Ferguson A. R., Sims A. P. 1974b; The regulation of glutamine metabolism in Candida utilis: the inactivation of glutamine synthetase. Journal of General Microbiology 80:173–185
    [Google Scholar]
  4. Foor F., Janssen K. A., Magasanik B. 1975; Regulation of synthesis of glutamine synthetase by adenylylated glutamine synthetase. Proceedings of the National Academy of Sciences of the United States of America 72:4844–4848
    [Google Scholar]
  5. Kohlaw G., Dragert W., Holzer H. 1965; Parallel Repression der Synthese von Glutamin-Synthese und DPN-abhangiger Glutamat-De-hydrogenase in Hefe. Biochemistry 341:224–238
    [Google Scholar]
  6. Kulka R. G., Cohen H. 1973; Regulation of glutamine synthetase activity of hepatoma tissue culture cells by glutamine and dexamethasone. Journal of Biological Chemistry 248:6738–6743
    [Google Scholar]
  7. Limon-Lason J., Lara M., Resendiz B., Mora J. 1977; Regulation of glutamine synthetase in fed-batch cultures of Neurospora crassa. Biochemical and Biophysical Research Communications 78:1234–1240
    [Google Scholar]
  8. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  9. Magasanik B., Prjval M., Brenchley J., Tyler B., De Leo A., Streicher S., Bender R., Paris C. G. 1974; Glutamine synthetase as a regulator of enzyme synthesis. Current Topics in Cellular Regulation 8:119–138
    [Google Scholar]
  10. McParland R. H, Guevara J. G., Becker R. R., Evans H. J. 1976; The purification and properties of the glutamine synthetase from the cytosol of soya-bean root nodules. Biochemical Journal 153:597–606
    [Google Scholar]
  11. Meister A. 1974; Glutamine synthetase of mammals. In The Enzymes vol.10: p. 699 Editor Boyer P. D. New York: Academic Press.;
    [Google Scholar]
  12. Milman G., Portnoff L. S., Tiemeier D. C. 1975; Immunochemical evidence for glutamine-mediated degradation of glutamine synthetase in cultured Chinese hamster cells. Journal of Biological Chemistry 250:1393–1399
    [Google Scholar]
  13. Mora Y., Espin G., Willms K., Mora J. 1978; Nitrogen accumulation in mycelium of Neurospora crassa. Journal of General Microbiology 104:241–250
    [Google Scholar]
  14. Moscona M., Frankel N., Moscona A. A. 1972; Regulatory mechanisms in the induction of glutamine synthetase in the embryonic retina: immunochemical studies. Developmental Biology 28:229–241
    [Google Scholar]
  15. Palacios R. 1976; Neurospora crassa glutamine synthetase. Purification by affinity chromatography and characterization of subunit structure. Journal of Biological Chemistry 251:4787–4791
    [Google Scholar]
  16. Palacios R., Campomanes M., Quinto C. 1977; Neurospora crassa glutamine synthetase. Translation of specific messenger ribonucleic acid in a cell-free system derived from rabbit reticulocytes. Journal of Biological Chemistry 252:3028–3034
    [Google Scholar]
  17. Pateman J. A. 1970; Regulation of synthesis of glutamate dehydrogenase and glutamine synthetase in microorganisms. Biochemical Journal 115:769–775
    [Google Scholar]
  18. Quinto C., Mora J., Palacios R. 1977; Neurospora crassa glutamine synthetase. Role of enzyme synthesis and degradation on the regulation of enzyme concentration during exponential growth. Journal of Biological Chemistry in the Press.
    [Google Scholar]
  19. Sanchez S., Martinez L., Mora J. 1972; Interactions between amino acid transport systems in Neurospora crassa. Journal of Bacteriology 112:276–284
    [Google Scholar]
  20. Sarkar P. K., Griffith B. 1976; Messenger RNA for glutamine synthetase: its partial purification, translation in a cell-free system and its regulation by hydrocortisone. Biochemical and Biophysical Research Communications 68:675–681
    [Google Scholar]
  21. Sims A. P., Toone J., Box V. 1974a; The regulation of glutamine synthesis in the food yeast Candida utilis: the purification and subunit structure of glutamine synthetase and aspects of enzyme deactivation. Journal of General Microbiology 80:485–499
    [Google Scholar]
  22. Sims A. P., Toone J., Box V. 1974b; The regulation of glutamine metabolism in Candida utilis: mechanisms of control of glutamine synthetase. Journal of General Microbiology 84:149–162
    [Google Scholar]
  23. Stadtman E. R., Ginsburg A. 1974; The glutamine synthetase of Escherichia coli structure and control. In The Enzymes vol.10: pp. 755–807 Editor Boyer P. D. New York: Academic Press.;
    [Google Scholar]
  24. Streicher S. L., Bender R. A., Magasanik B. 1975; Genetic control of glutamine synthetase in Klebsiella aerogenes. Journal of Bacteriology 121:320–331
    [Google Scholar]
  25. Tiemeier D. C., Milman G. 1972; Regulation of glutamine synthetase in cultured Chinese hamster cells. Journal of Biological Chemistry 247:5722–5727
    [Google Scholar]
  26. Tyler B., De Leo A., Magasanik B. 1974; Activation of transcription of hut DNA by glutamine synthetase. Proceedings of the National Academy of Sciences of the United States of America 71:225–229
    [Google Scholar]
  27. Vogel H. J. 1964; Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98:435–446
    [Google Scholar]
  28. Weglenski P., Tyler B. 1977; Regulation of gin A messenger ribonucleic acid synthesis in Klebsiella aerogenes. Journal of Bacteriology 129:880–887
    [Google Scholar]
  29. WOHLHUETER R. M., SCHUTT H., HOLZER H.D. 1973; Regulation of glutamine synthetase in vivo in Escherichia coli. In The Enzymes of Glutamine Metabolism pp 45–64 Editor Prusiner S., Stadtman E. R. New York: Academic Press.;
    [Google Scholar]
  30. Yemm E. W., Cocking E. C. 1955; The determination of amino acids with ninhydrin. Analyst 80:209–214
    [Google Scholar]
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