1887

Abstract

Methods for isolating mutants of 8250 constitutive for the mandelate enzymes were compared. Non-inducing substrates were not available. Continuous culture gave no stable constitutive mutants. Alternate culture in inducing (-mandelate) and non-inducing (-glutamate) media led to the isolation of mutants mesoconstitutive for -mandelate dehydrogenase and phenylglyoxylate carboxy-lyase, and hyperinducible for benzaldehyde dehydrogenase I. Screening of possible anti-inducers showed that 2-phenyl-propionate was very effective; it was a competitive inhibitor of gratuitous induction by thiophenoxyacetate, and was used for the isolation of constitutive mutants. Some of the mutants were magnoconstitutive for -mandelate dehydrogenase, phenylglyoxylate carboxylyase and benzaldehyde dehydrogenase I and could not be further induced; others had lower specific activities which could be increased by induction with phenylglyoxylate or thiophenoxyacetate. Similar constitutive mutants were derived from a mutant which lacked -mandelate dehydrogenase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-106-2-233
1978-06-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/106/2/mic-106-2-233.html?itemId=/content/journal/micro/10.1099/00221287-106-2-233&mimeType=html&fmt=ahah

References

  1. Beggs J. D., Fewson C. A. 1977; Regulation of synthesis of benzyl alcohol dehydrogenase in Acinetobacter calcoaceticus ncib 8250. Journal of General Microbiology 103:127–140
    [Google Scholar]
  2. Beggs J. D., Cook A. M., Fewson C. A. 1976; Regulation of growth of Acinetobacter calcoaceticus ncib 8250 on benzyl alcohol in batch culture. Journal of General Microbiology 96:365–374
    [Google Scholar]
  3. Brammar W. J., Clarke P. H., Skinner A. J. 1967; Biochemical and genetic studies with regulator mutants of the Pseudomonas aeruginosa 8602 amidase system. Journal of General Microbiology 47:87–102
    [Google Scholar]
  4. Cohen-Bazere G., Jolit M. 1953; Isolement par selection de mutants d'Escherichia coli synth6-tisant spontanement Pamylomaltase et la-galactosidase. Annales de VInstitut Pasteur 84:937–945
    [Google Scholar]
  5. Cook A. M., Beggs J. D., Fewson C. A. 1975; Regulation of growth of Acinetobacter calcoaceticus ncib 8250 on L-mandelate in batch culture. Journal of General Microbiology 91:325–337
    [Google Scholar]
  6. Fewson C. A. 1967a; The growth and metabolic versatility of the Gram-negative bacterium ncib 8250 (Vibrio 01). Journal of General Microbiology 46:255–266
    [Google Scholar]
  7. Fewson C. A. 1967b; The identity of the Gram-negative bacterium ncib 8250 (Vibrio 01). Journal of General Microbiology 48:107–110
    [Google Scholar]
  8. Fewson C. A., Foote A. 1976; Inhibition of induction of the mandelate regulon in Acinetobacter calcoaceticus ncib 8250. Proceedings of the Society for General Microbiology 3:177
    [Google Scholar]
  9. Hegeman G. D. 1966; Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. III. Isolation and properties of constitutive mutants. Journal of Bacteriology 91:1161–1167
    [Google Scholar]
  10. Hegeman G. D., Root R. T. 1976; The effect of a non-metabolizable analog on mandelate catabolism in Pseudomonas putida. Archives of Microbiology 110:19–25
    [Google Scholar]
  11. Higgins S. J., Mandelstam J. 1972; Regulation of pathways degrading aromatic substrates in Pseudomonas putida. Enzyme response to binary mixtures of substrates. Biochemical Journal 126:901–916
    [Google Scholar]
  12. Kennedy S. I. T., Fewson C. A. 1968a; Enzymes of the mandelate pathway in bacterium ncib 8250. Biochemical Journal 107:497–506
    [Google Scholar]
  13. Kennedy S. I. T., Fewson C. A. 1968b; Metabolism of mandelate and related compounds by bacterium ncib 8250. Journal of General Microbiology 53:259–273
    [Google Scholar]
  14. Livingstone A. 1970; Induction and activity of the mandelate pathway enzymes in bacterium ncib 8250. Ph.D. thesis University of Glasgow.;
    [Google Scholar]
  15. Livingstone A., Fewson C. A. 1972; Regulation of the enzymes converting L-mandelate into benzoate in bacterium ncib 8250. Biochemical Journal 130:937–946
    [Google Scholar]
  16. Livingstone A., Fewson C. A., Kennedy S. I. T., Zatman L. J. 1972; Two benzaldehyde dehydrogenases in bacterium ncib 8250. Distinguishing properties and regulation. Biochemical Journal 130:927–935
    [Google Scholar]
  17. Moyes H. M., Fewson C. A. 1976; Consti-tutivity of the mandelate enzymes in Acinetobacter calcoaceticus ncib 8250, and its effect on the synthesis of benzyl alcohol dehydrogenase. Biochemical Society Transactions 4:1105–1106
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-106-2-233
Loading
/content/journal/micro/10.1099/00221287-106-2-233
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error