RT Journal Article SR Electronic(1) A1 Bourguignon, Lilly Y. W. A1 Katz, E. R.YR 1978 T1 Isolation and Characterization of the RNA of Membrane-bound Ribosomes in Dictyostelium discoideum JF Microbiology, VO 106 IS 1 SP 93 OP 101 DO https://doi.org/10.1099/00221287-106-1-93 PB Microbiology Society, SN 1465-2080, AB The RNA of membrane-bound ribosomes, isolated from Dictyostelium discoideum, represented 13 to 16% of the total ribosomal RNA (rRNA) present throughout growth and development. Membrane-bound ribosomes were released by treatment with sodium deoxycholate and Brij 58. There were no obvious differences in size and base composition between RNAs derived from membrane-bound or free ribosomes. The 17S membrane-bound rRNA and free rRNAs appeared to have similar methyl contents. However, the 25S membrane-bound rRNA contained about 16 to 20% fewer methyl groups than the 17S membrane-bound rRNA and free rRNAs. Free rRNAs turned over rapidly during early development but not during the disaggregation and reaggregation processes. Membrane-bound rRNAs showed very little turnover during the early stages of morphogenesis, but showed rapid turnover during the late stages of development; this class of rRNAs did not turn over during early stages of reaggregation but turned over rapidly during later stages of reaggregation., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-106-1-93