%0 Journal Article %A Stetler, Dean A. %A Boguslawski, George %A Decedue, Charles J. %T Effect of Elevated Temperature on Growth and Ribonucleic Acid Synthesis in Cryptococcus albidus %D 1978 %J Microbiology, %V 106 %N 1 %P 67-72 %@ 1465-2080 %R https://doi.org/10.1099/00221287-106-1-67 %I Microbiology Society, %X When a growing culture of Cryptococcus albidus was shifted from 27 to 37 °C, growth ceased abruptly and the cells died rapidly (over 90% after 4 h). The temperature shift resulted in a rapid cessation of [3H]uridine incorporation (as perchloric acid-insoluble material). DEAE-Sephadex chromatography of crude extract from C. albidus cells revealed the presence of two major enzyme fractions with RNA polymerase activity. The fractions were insensitive to α-amanitin, and both synthesized RNA efficiently at 23 °C but not at 37 °C. Neither proteolytic digestion of the enzymes nor ribonuclease degradation of the RNA products could account for the observed decline in RNA synthesis at the elevated temperature. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-106-1-67