When a growing culture of was shifted from 27 to 37 °C, growth ceased abruptly and the cells died rapidly (over 90% after 4 h). The temperature shift resulted in a rapid cessation of [H]uridine incorporation (as perchloric acid-insoluble material). DEAE-Sephadex chromatography of crude extract from cells revealed the presence of two major enzyme fractions with RNA polymerase activity. The fractions were insensitive to α-amanitin, and both synthesized RNA efficiently at 23 °C but not at 37 °C. Neither proteolytic digestion of the enzymes nor ribonuclease degradation of the RNA products could account for the observed decline in RNA synthesis at the elevated temperature.


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