1887

Abstract

Respiration rates, adenine nucleotide levels and heat production were measured during exponential asynchronous growth of and in synchronous cultures prepared by continuous-flow selection. In synchronous cultures rates of oxygen uptake rose to maxima three times in each cell cycle. The ATP pool oscillated in phase with respiratory activity. The ADP and AMP pools oscillated in phase with each other, but out of phase with ATP, and the rate of heat production also showed fluctuations. Values calculated for adenylate energy charge were low, increasing from less than 0.2 to more than 0.4 during the mid-exponential phase of growth in an asynchronous culture, and oscillating over a range from 0.34 to 0.47 in a synchronous culture.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-106-1-19
1978-05-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/106/1/mic-106-1-19.html?itemId=/content/journal/micro/10.1099/00221287-106-1-19&mimeType=html&fmt=ahah

References

  1. Atkinson D. E., Walton G. M. 1967; Adenosine triphosphate conservation in metabolic regulation. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  2. Ball W. J., Atkinson D. E. 1975; Adenylate energy charge in Saccharomyces cerevisiae during starvation. Journal of Bacteriology 121:975–982
    [Google Scholar]
  3. Blumenthal L. K., Zahler S. A. 1962; Index for measurement of synchronization of cell populations. Science 135:724
    [Google Scholar]
  4. Chance B., Williams G. R. 1956; The respiratory chain and oxidative phosphorylation. Advances in Enzymology 17:65–134
    [Google Scholar]
  5. Chapman A. G., Atkinson D. E. 1977; Adenine nucleotide concentrations and turnover rates. Their correlation with biological activity in bacteria and yeast. Advances in Microbial Physiology 15253–306
    [Google Scholar]
  6. Chapman A. G., Fall L., Atkinson D. E. 1971; Adenylate energy charge in Escherichia coli during growth and starvation. Journal of Bacteriology 108:1072–1086
    [Google Scholar]
  7. De Balros A. V., De Castro J. F., De Castro F. J. 1973; The effect of temperature on nucleotide pool formation in Tetrahymena pyriformis. Journal of Cellular Physiology 81:149–152
    [Google Scholar]
  8. Dietzler D. N., Lais C. J., Magnani J. L., Leckie M. P. 1974; Maintenance of the energy charge in the presence of large decreases in the total adenylate pool of Escherichia coli and concurrent changes in glucose-6-P, fructose-P2 and glycogen synthesis. Biochemical and Biophysical Research Communications 60:875–881
    [Google Scholar]
  9. Edwards S. W., Lloyd D. 1977; Changes in oxygen uptake rates, enzyme activities, cytochrome amounts and adenine nucleotide pool levels during growth of Acanthamoeba castellanii in batch culture. Journal of General Microbiology 102:135–144
    [Google Scholar]
  10. Edwards C., Statham M., Lloyd D. 1975; The preparation of large-scale synchronous cultures of the trypanosomatid, Crithidia fasciculata, by cell-size selection: changes in respiration and adenylate charge through the cell-cycle. Journal of General Microbiology 88:141–152
    [Google Scholar]
  11. Knowles C. J. 1977; Microbial metabolic regulation by adenine nucleotide pools. Symposia of the Society for General Microbiology 27: [View Article][PubMed]
    [Google Scholar]
  12. Lloyd D. 1974; Environmental modification of mitochondrial composition and activities. In The Mitochondria of Microorganisms pp 159–250 London: Academic Press.;
    [Google Scholar]
  13. Lloyd D., Brightwell R., Venables S. E., Roach G. I., Turner G. 1971; Subcellular fractionation of Tetrahymena pyriformis ST by zonal centrifugation: changes in activities and distribution of enzymes during the growth cycle and on starvation. Journal of General Microbiology 65:209–223
    [Google Scholar]
  14. Lloyd D., John L., Edwards C., Chagla A. H. 1975; Synchronous cultures of microorganisms : large-scale preparation by continuous-flow size selection. Journal of General Microbiology 88:153–158
    [Google Scholar]
  15. Lloyd D., Morgan N. A., John L., Venables S. E. 1978; Starvation of Prototheca zopfii. Journal of General Microbiology 105:1–10
    [Google Scholar]
  16. Lovlie A. 1963; Growth in mass and respiration rate during the cell cycle of Tetrahymena pyriformis. Comptes Rendus des travaux du Laboratoire Carlsberg 33:377–413
    [Google Scholar]
  17. Niven D. F., Collins P. A., Knowles C. J. 1977; Adenylate energy charge during batch culture of Beneckea natriegens. Journal of General Microbiology 98:95–108
    [Google Scholar]
  18. Phillips C. A., Lloyd D. 1978; Continuous-flow size selection of Tetrahymena pyriformis strain st: changes in volume, DNA, RNA and protein during synchronous growth. Journal of General Microbiology 105:95–103
    [Google Scholar]
  19. Plesner P. 1964; Nucleotide metabolism during synchronized cell division in Tetrahymena pyriformis. Comptes Rendus des travaux du Laboratoire Carlsberg 34:1–76
    [Google Scholar]
  20. Poole R. K., Lloyd D., Kemp R. B. 1973; Respiratory oscillations and heat evolution in synchronously dividing cultures of the fission yeast Schizosaccharomyces pombe 972h. Journal of General Microbiology 77:209–220
    [Google Scholar]
  21. Scherbaum O. H., Chou S. C., Seraydarian K. H., Byfield J. E. 1962; The effect of temperature shifts on the intracellular level of nucleoside triphosphates in Tetrahymena pyri-formis. Canadian Journal of Microbiology 8: [View Article][PubMed]
    [Google Scholar]
  22. Stocco D. M., Zimmerman A. M. 1975; Adenine nucleotide metabolism in heat-synchronized Tetrahymena. Molecular and Cellular Biochemistry 1:187–194
    [Google Scholar]
  23. Swedes J. S., Sedo R. J., Atkinson D. E. 1975; Relation of growth and protein synthesis to the adenylate energy charge in an adenine-requiring mutant of Escherichia coli. Journal of Biological Chemistry 250:6930–6938
    [Google Scholar]
  24. Zeuthen E., Scherbaum O. 1954; Synchronous divisions in mass cultures of the ciliate protozoan Tetrahymena pyriformis as induced by temperature changes. In Recent Developments in Cell Physiology. Proceedings of the 7th Symposium of the Colston Research Society pp. 141–156 Editor Kitching J. A. London: Butterworths;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-106-1-19
Loading
/content/journal/micro/10.1099/00221287-106-1-19
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error