1887

Abstract

The contribution of phagocytes to protection against was analysed in outbred ddN mice. Most of the bacteria injected intravenously at a dose of 3 × 10 to 4 × 10 were trapped in the liver within 10 min. There was a transient 10-fold decrease in the number of bacteria by 6 h. Anti-listeria activity in the initial phase was resistant to X-irradiation but was inhibited by carrageenan, and was not influenced by immunization. The protection in this very early stage of infection seemed to be attributable to the function of fixed macrophages. Viable bacteria in the organs increased progressively but slowly from 6 h to 72 h to reach maximum numbers. Bacterial growth during this period was markedly enhanced by X-irradiation or treatment with carrageenan. Accumulation of free phagocytes seemed to suppress the bacterial growth in this phase. The number of bacteria began to decrease from day 4 and became undetectable by day 9. The suppressive effect on bacterial growth in this last phase may be dependent on immunologically activated macrophages and was reversed by X-irradiation and carrageenan. The course of local infection was similar to that of systemic infection except for the lack of initial decrease. We conclude that the course of infection with can be divided into three phases with regard to the roles of phagocytes in resistance.

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/content/journal/micro/10.1099/00221287-106-1-165
1978-05-01
2021-05-16
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References

  1. Allison A. C., Harington J. S., Birbeck M. 1966; An examination of the cytotoxic effects of silica on macrophages. Journal of Experimental Medicine 124:141–153
    [Google Scholar]
  2. Benacerraf B., , Kivy- Rosenberg E., Sebestyen M. M., Zweifach B. W. 1959; The effect of high doses of X-irradiation on the phagocytic, proliferative and metabolic properties of the reticuloendothelial system. Journal of Experimental Medicine 110:49–63
    [Google Scholar]
  3. Catanzaro P. J., Schwartz H. J., Graham R. C. 1971; Spectrum and possible mechanism of carrageenan cytotoxicity. American Journal of Pathology 64:387–404
    [Google Scholar]
  4. Emmerling P., Finger H., Bockemuhl J. 1975; Listeria monocytogenes infection in nude mice. Infection and Immunity 12:437–439
    [Google Scholar]
  5. Gillette R. W., Lance E. M. 1973; Kinetic studies of macrophages. IV. Effect of irradiation. Journal of the Reticuloendothelial Society 14:18–25
    [Google Scholar]
  6. Korneeld L., Greenman V. 1966; Effect of total body X-irradiation on peritoneal cells of mice. Radiation Research 29:433–444
    [Google Scholar]
  7. Mackaness G. B. 1962; Cellular resistance to infection. Journal of Experimental Medicine 116:381–406
    [Google Scholar]
  8. Mackaness G. B. 1969; The influence of immunologically committed lymphoid cells on macrophage activity in vivo. Journal of Experimental Medicine 129:973–992
    [Google Scholar]
  9. McGregor D. D., Koster F. T. 1971; The mediator of cellular immunity. IV. Cooperation between lymphocytes and mononuclear phagocytes. Cellular Immunology 2:317–325
    [Google Scholar]
  10. Nelson D. S. 1969; Macrophage and Immunity. Amsterdam: North Holland Publishing Company;
    [Google Scholar]
  11. North R. J. 1969; Cellular kinetics associated with the development of acquired cellular resistance. Journal of Experimental Medicine 130:299–314
    [Google Scholar]
  12. North R. J. 1970; The relative importance of blood monocytes and fixed macrophages to the expression of cell-mediated immunity to infection. Journal of Experimental Medicine 132:521–534
    [Google Scholar]
  13. Takeya K., Shimotori S., Taniguchi T., Nomoto K. 1977; Cellular mechanisms in the protection against infection by Listeria monocytogenes in mice. Journal of General Microbiology 100:373–379
    [Google Scholar]
  14. Tripathy S. P., Mackaness G. B. 1969; The effect of cytotoxic agents on the primary immune response to Listeria monocytogenes. Journal of Experimental Medicine 130:1–16
    [Google Scholar]
  15. Volkman A., Collins F. M. 1971; The restorative effect of peritoneal macrophages on delayed hypersensitivity following ionizing radiation. Cellular Immunology 2:552–566
    [Google Scholar]
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