%0 Journal Article %A Boylen, Charles W. %A Mulks, Martha H. %T The Survival of Coryneform Bacteria during Periods of Prolonged Nutrient Starvation %D 1978 %J Microbiology, %V 105 %N 2 %P 323-334 %@ 1465-2080 %R https://doi.org/10.1099/00221287-105-2-323 %I Microbiology Society, %X Summary: Cultures of 16 coryneform bacteria were grown to late-exponential stage in nutrient media, washed, and starved in 30 mm-potassium phosphate buffer pH 7.0, with no external energy or carbon source. After 4 weeks starvation, 20 to 98% of each culture was still viable; after 8 weeks, 5 to 70% of each culture was still viable. Little change in cell shape or size was detected in Arthrobacter globiformis, A. nicotianae, Brevibacterium linens, Corynebacterium fascians, Mycobacterium rhodochrous and Nocardia roseum when studied by electron microscopy for up to 56 d, although there was a gradual disappearance of intracellular material. No resting structures were discernible. All organisms showed an immediate decrease in endogenous respiration to less than 1% of that observed during growth. A low basal level of endogenous metabolism equivalent to 0.01 to 0.03% of cellular carbon oxidized to CO2 h-1 was maintained for 56 d. Carbohydrate, intracellular pools, protein, ribonucleic acid and deoxyribonucleic acid were utilized at varying rates by different organisms during this period. All species were effective in maintaining 20 to 70% of their Mg2+ content during a 28 d starvation period in the absence of any external Mg2+. It would appear that the soil coryneform bacteria possess similar survival characteristics in laboratory studies which could explain, in part, their ecological success in natural environments. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-105-2-323