1887

Abstract

Summary: Sucrose catabolism was studied in Sucrose was hydrolysed by the action of a constitutive cytoplasmic sucrase. The use of a glucose-6-phosphate dehydro-genase-deficient mutant and radiorespirometric experiments demonstrated that both the glucose and the fructose moieties of sucrose were catabolized via the Entner-Doudoroff pathway. This result was confirmed by enzyme analysis and studies on sugar assimilation. All the enzymes of the Entner-Doudoroff pathway were present in bacteria grown on sucrose but fructokinase (EC 2.7.1.4) activity was relatively low. In contrast, phosphoenol-pyruvate: fructose phosphotransferase and 1-phosphofructokinase, the key enzymes for the catabolism of exogenous fructose, were only partially induced. Bacteria grown on sucrose and treated with chloramphenicol were, therefore, not able to assimilate exogenous fructose. We conclude that under these conditions endogenous fructose is catabolized via the Entner-Doudoroff pathway, while exogenous fructose is degraded via fructose 1-phosphate and the Embden-Meyerhof pathway.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-105-2-305
1978-04-01
2021-05-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/105/2/mic-105-2-305.html?itemId=/content/journal/micro/10.1099/00221287-105-2-305&mimeType=html&fmt=ahah

References

  1. Anderson R. L., Kamel M. Y. 1966; Acyl-phosphate :hexose phosphotransferase (hexose-phosphate:hexose phosphotransferase). Methods in Enzymology 9:392–396
    [Google Scholar]
  2. Baumann P., Baumann L. 1975; Catabolism of d-fructose and D-ribose by Pseudomonas doudoroffii. I. Physiological studies and mutant analysis. Archives of Microbiology 105:225–240
    [Google Scholar]
  3. Chassy B. M., Krichevsky M. J. 1972; Synthesis of glucose-l-phosphate-U-14C and specifically labelled sucrose using sucrose phosphorylase. Analytical Biochemistry 49:232–239
    [Google Scholar]
  4. Conrad R., Schlegel H. G. 1974; Different pathways for fructose and glucose utilization in Rhodopseudomonas capsulata and demonstration of 1-phosphofructokinase in phototrophic: bacteria. Biochimica et biophysica acta 358:221–225
    [Google Scholar]
  5. Conrad R., Schlegel H. G. 1977a; Different degradation pathways for glucose and fructose in Rhodopseudomonas capsulata . Archives of Microbiology 112:39–48
    [Google Scholar]
  6. Conrad R., Schlegel H. G. 1977b; Influence of aerobic and phototrophic growth conditions on the distribution of glucose and fructose carbon into the Entner-Doudoroff and the Embden-Meyerhof pathways in Rhodopseudomonas sphae-roides . Journal of General Microbiology 101:277–290
    [Google Scholar]
  7. Delobbe A., Chalumeau H., Gay P. 1975; Existence of two alternative pathways for fructose and sorbitol metabolism in Bacillus subtilis Marburg. European Journal of Biochemistry 51:503–510
    [Google Scholar]
  8. Dische Z. 1962; Colour reactions of hexoses. Methods in Carbohydrate Chemistry 1:488–494
    [Google Scholar]
  9. Eidels L., Preiss J. 1970; Carbohydrate meta-bolism in Rhodopseudomonas capsulata: enzyme titers, glucose metabolism, and polyglucose polymer synthesis. Archives of Biochemistry and Biophysics 140:75–89
    [Google Scholar]
  10. Ferenci T., Kornberg H. L. 1971; Pathway of fructose utilization by Escherichia coli . FEBS Letters 13:127–130
    [Google Scholar]
  11. Ferenci T., Kornberg H. L. 1973; The utilization of fructose by Escherichia coli. Properties of a mutant defective in fructose 1-phosphate kinase activity. Biochemical Journal 132:341–347
    [Google Scholar]
  12. Fraenkel D. G. 1968; The phosphoenolpyru-vate-initiated pathway of fructose metabolism in Escherichia coli . Journal of Biological Chemistry 243:6458–6463
    [Google Scholar]
  13. Fukui K., Fukui Y., Moriyama T. 1974; Purification and properties of dextransucrase and invertase from Streptococcus mutans . Journal of Bacteriology 118:796–804
    [Google Scholar]
  14. Gay P., Rapoport G. 1970; Étude des mutants depourvus de fructose-1-phosphate kinase chez Bacillus subtilis . Comptes rendus hebdomadaires des seances de l’Acadeémie des sciences,Série D :Sciences Naturelles 271:374–377
    [Google Scholar]
  15. Hengstenberg W., Egan J. B., Morse M. L. 1968; Carbohydrate transport in Staphylococcus aureus. VI. The nature of the derivatives accumulated. Journal of Biological Chemistry 243:1881–1885
    [Google Scholar]
  16. Janda S., Hedenström. 1974; Uptake of di-saccharides by the aerobic yeast Rhodotorula glutinis. Hydrolysis of β-fructosides and trehalose. Archives of Microbiology 101:273–280
    [Google Scholar]
  17. Karunairatnam M. C., Spizizen J., Gest H. 1958; Preparation and properties of protoplasts of Rhodospirillum rubrum . Biochimica et biophysica acta 29:649–650
    [Google Scholar]
  18. Kelker N. E., Hanson T. E., Anderson R. L. 1970; Alternate pathways of d-fructose metabolism in Aerobacter aerogenes. A specific d-fructokinase and its preferential role in the metabolism of sucrose. Journal of Biological Chemistry 245:2060–2065
    [Google Scholar]
  19. Kidby D. K., Davies R. 1970; Thiol induced release of invertase from cell walls of Saccharo-myces fragilis . Biochimica et biophysica acta 201:261–266
    [Google Scholar]
  20. Laishley E. J. 1975; Regulation and properties of an invertase from Clostridium pasteurianum . Canadian Journal of Microbiology 21:1711–1718
    [Google Scholar]
  21. Lepesant J. -A., Dedonder R. 1968; Transport du saccharose chez Bacillus subtilis . Comptes rendus hebdomadaires des séances de VAcademie des sciences, Série D: Sciences Naturelles 267:1109–1112
    [Google Scholar]
  22. Lepesant J. -A., Kunst F., Lepesant-Kejzela-Rova J., Dedonder R. 1972; Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg. Molecular and General Genetics 118:135–160
    [Google Scholar]
  23. Metzenberg R. L. 1963; The localization of β-fructofuranosidase in Neurospora . Biochimica et biophysica acta 77:455–465
    [Google Scholar]
  24. Pascal M., Kunst F., Lepesant J. -A., Dedonder R. 1971; Characterization of two sucrase activities in Bacillus subtilis Marburg. Biochimie 53:1059–1066
    [Google Scholar]
  25. Patni N. J., Alexander J. K. 1971; Catabolism of fructose and mannitol in Clostridium thermo-cellum: presence of phosphoenolpyruvate: fructose phosphotransferase, fructose-1-phosphate kinase, phosphoenolpyruvate:mannitol phospho-transferase, and mannitol-l-phosphate dehydro-genase in cell extracts. Journal of Bacteriology 105:226–231
    [Google Scholar]
  26. Phibbs P. V. Jr Mccowen S. M., Feary T. W., Blevins W. T. 1978; Mannitol and fructose catabolic pathways of carbohydrate-negative mutant strains of Pseudomonas aeruginosa and pleiotropic effects of certain enzyme deficiencies. Journal of Bacteriology in the Press
    [Google Scholar]
  27. Prestidge L. S., Spizizen J. 1969; Inducible sucrase activity in Bacillus subtilis distinct from levan-sucrase. Journal of General Microbiology 59:285–288
    [Google Scholar]
  28. Sawyer M. H., Baumann P., Baumann L., Berman S. M., Cánovas J. L., Berman R. H. 1977a; Pathways of d-fructose catabolism in species of Pseudomonas . Archives of Microbiology 112:49–55
    [Google Scholar]
  29. Sawyer M. H., Baumann P., Baumann L. 1977b; Pathways of d-fructose and d-glucose catabolism in marine species of Alcaligenes, Pseudomonas marina and Alteromonas communis . Archives of Microbiology 112:169–172
    [Google Scholar]
  30. Schmidt K., Liaaen-Jensen S., Schlegel H. G. 1963; Die Carotinoide der Thiorhodaceae. I. Okenon als Hauptcarotinoid von Chromatium okenii Perty. Archiv für Mikrobiologie 46:117–126
    [Google Scholar]
  31. Sutton D. D., Lampen J. O. 1962; Localization of sucrose and maltose fermenting systems in Saccharomyces cerevisiae . Biochimica et bio-physica acta 56:303–312
    [Google Scholar]
  32. Tanzer J. M., Brown A. T., Mcinerney M. F. 1973; Identification, preliminary characteriza-tion and evidence for regulation of invertase in Streptococcus mutans . Journal of Bacteriology 116:192–202
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-105-2-305
Loading
/content/journal/micro/10.1099/00221287-105-2-305
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error