1887

Abstract

Summary: Sucrose catabolism was studied in Sucrose was hydrolysed by the action of a constitutive cytoplasmic sucrase. The use of a glucose-6-phosphate dehydro-genase-deficient mutant and radiorespirometric experiments demonstrated that both the glucose and the fructose moieties of sucrose were catabolized via the Entner-Doudoroff pathway. This result was confirmed by enzyme analysis and studies on sugar assimilation. All the enzymes of the Entner-Doudoroff pathway were present in bacteria grown on sucrose but fructokinase (EC 2.7.1.4) activity was relatively low. In contrast, phosphoenol-pyruvate: fructose phosphotransferase and 1-phosphofructokinase, the key enzymes for the catabolism of exogenous fructose, were only partially induced. Bacteria grown on sucrose and treated with chloramphenicol were, therefore, not able to assimilate exogenous fructose. We conclude that under these conditions endogenous fructose is catabolized via the Entner-Doudoroff pathway, while exogenous fructose is degraded via fructose 1-phosphate and the Embden-Meyerhof pathway.

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/content/journal/micro/10.1099/00221287-105-2-305
1978-04-01
2019-10-16
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-105-2-305
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