Summary: In contrast to observations by previous workers of an inverse relationship between inoculum size and the length of the lag phase, the lag time of was found to be independent of inoculum size whether the inocula were derived from an exponential phase, diauxic lag phase or stationary phase parent culture. It is concluded that the lag phase of this organism is dependent on the internal physiological state of the parent culture cells, rather than the need to accumulate diffusible substances in the extracellular medium.


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