RT Journal Article SR Electronic(1) A1 Hanson, Maureen R. A1 Bogorad, LawrenceYR 1978 T1 The ery-M2 Group of Chlamydomonas reinhardii: Cold-sensitive, Erythromycin-resistant Mutants Deficient in Chloroplast Ribosomes JF Microbiology, VO 105 IS 2 SP 253 OP 262 DO https://doi.org/10.1099/00221287-105-2-253 PB Microbiology Society, SN 1465-2080, AB Summary: Erythromycin-resistant mutants mapping to the Mendelian ery-M2 locus of Chlamydomonas reinhardii were cold-sensitive for growth at 15 °C. The phenotypes erythromycin-resistance and cold-sensitivity could not be separated in crosses. Mutants at the ery-M2 locus were previously found to have chloroplast ribosomes with altered [14C]erythromycin binding capacity ( Mets & Bogorad, 1971 ). When grown at 25 °C, haploid strains containing ery-M2 alleles alone or in combination with other ery r loci had fewer chloroplast ribosomes than the wild type. A further reduction occurred when ery-M2 strains were shifted to 15 °C. Diploids containing both ery-M2 and its wild-type allele contained normal amounts of chloroplast ribosomes at 25 and 15°C, and were cold-resistant and antibiotic-sensitive. The inability of chloroplast ribosome-deficient ery-M2 strains to grow at 15 °C in the presence of a reduced carbon source indicates that chloroplast protein synthesis is essential for cellular growth even when photosynthesis is not essential., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-105-2-253