1887

Abstract

The passive permeation and facilitated diffusion of glycerol in various strains of have been studied by stopped-flow spectrophotometry. Contrary to the prediction for glycerol entry by simple diffusion, the reciprocal relaxation time (1/𝜏, s) for the passive permeation of glycerol in cells grown in the presence of glucose was not constant but decreased as the glycerol concentration increased above 100 mm. This anomaly was not due to refractive index differences or to the presence of residual levels of the glycerol facilitator protein in non-induced cells. Although reciprocal relaxation times for glycerol-induced exhibited the expected elevation relative to non-induced cells, a similar anomalous decrease of 1/𝜏 (s) with increasing glycerol concentration was observed. In addition, at early times after suspension in dilute buffer, the 1/𝜏 (s) values obtained for induced or non-induced swelling in glycerol were considerably greater than for organisms incubated in dilute buffer for longer times. We concluded that either this spectro-photometric technique was not monitoring solely the permeation of glycerol into , or concentrations of glycerol above 100 mm significantly perturbed the structure of the cell envelope.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-105-2-233
1978-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/105/2/mic-105-2-233.html?itemId=/content/journal/micro/10.1099/00221287-105-2-233&mimeType=html&fmt=ahah

References

  1. Alemohammad M.M., Knowles C.J. 1974; Osmotically induced volume and turbidity changes of Escherichia coli due to salts, sucrose and glycerol, with particular reference to the rapid permeation of glycerol into the cell. Journal of General Microbiology 82:125–142
    [Google Scholar]
  2. Bangham A.D., De Gier J., Greville G.D. 1967; Osmotic properties and water permeability of phospholipid liquid crystals. Chemistry and Physics of Lipids 1:225–246
    [Google Scholar]
  3. Cozzarelli N.R., Freedberg W.B., Lin E.C.C. 1968; Genetic control of the l-Ξ±-glycero-phosphate system in Escherichia coli.. Journal of Molecular Biology 31:371–387
    [Google Scholar]
  4. Daniels F., Alberty R.A. 1966; Chemical kinetics. In Physical Chemistry, 3rd edn. pp. 325–330 New York: John Wiley;
    [Google Scholar]
  5. De Gier J., Mandersloot J.G., Van Deenen L.L.M. 1968; Lipid composition and permeability of liposomes. Biochimica et biophysica acta 150:666–675
    [Google Scholar]
  6. De Gier J., Mandersloot J.G., Hupkes J.V., Mcelhaney R.N., Van Beek W.P. 1971; On the mechanism of non-electrolyte permeation through lipid bilayers and through biomembranes. Biochimica et biophysica acta 233:610–618
    [Google Scholar]
  7. Gibson Q.H., Milnes L. 1964; Apparatus for rapid and sensitive spectrophotometry. Biochemical Journal 91:161–171
    [Google Scholar]
  8. Hague D.N. 1969; Experimental methods for the study of fast reactions. In Comprehensive Chemical Kinetics 1 The Practice of Kinetics pp. 112–133 Edited by Bamford C.H., Tipper C.F.H. Amsterdam: Elsevier;
    [Google Scholar]
  9. Langdon R.G. 1966; Glucose-6-phosphate dehydrogenase from erythrocytes. Methods in Enzymology ix:126–127
    [Google Scholar]
  10. Maloney P.C., Kashket E.R., Wilson T.H. 1975; Methods for studying transport in bacteria. In Methods in Membrane Biology 5 pp. 1–49 Edited by Korn E.D. New York: Plenum Press;
    [Google Scholar]
  11. Mcelhaney R.N., De Gier J., Van Deenen L.L.M. 1970; The effect of alterations in fatty acid composition and cholesterol content on the permeability of Mycoplasma laidlawii B cells and derived liposomes. Biochimica et biophysica acta 219:245–247
    [Google Scholar]
  12. Mcelhaney R.N., De Gier J., Van Der Neut-Kok E.C.M. 1973; The effect of altera-tions in fatty acid composition and cholesterol content on the nonelectrolyte permeability of Acholeplasma laidlawii B cells and derived liposomes. Biochimica et biophysica acta 298:500–512
    [Google Scholar]
  13. Miller J. 1972 Experiments in Molecular Genetics New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Mitchell P., Moyle J. 1956; Osmotic function and structure in bacteria. Symposia of the Society for General Microbiology 6:150–180
    [Google Scholar]
  15. Netter H. 1969; Osmosis. In Theoretical Biochemistry: Physico-chemical Principles of Vital Processes English translation pp. 102–103 New York: John Wiley;
    [Google Scholar]
  16. Read B.D., Mcelhaney R.N. 1975; Glucose transport in Acholeplasma laidlawii B: dependence on the fluidity and physical state of membrane lipids. Journal of Bacteriology 123:47–55
    [Google Scholar]
  17. Richey D.P., Lin E.C.C. 1972; Importance of facilitated diffusion for effective utilization of glycerol by Escherichia coli.. Journal of Bacteriology 112:784–790
    [Google Scholar]
  18. Sanno Y., Wilson T.H., Lin E.C.C. 1968; Control of permeation to glycerol in cells of Escherichia coli.. Biochemical and Biophysical Research Communications 32:344–349
    [Google Scholar]
  19. Shechter E., Letellier L., Gulik-Krzywicki T. 1974; Relations between structure and function in cytoplasmic membrane vesicles isolated from an Escherichia coli fatty acid auxo-troph: high-angle X-ray diffraction, freeze-etch electron microscopy and transport studies. European Journal of Biochemistry 49:61–76
    [Google Scholar]
  20. Stein W.D. 1958; N-terminal histidine at the active centre of a permeability mechanism. Nature; London: 1811662–1663
    [Google Scholar]
  21. Stein W.D. 1962; Spontaneous and enzyme-induced dimer formation and its role in membrane permeability.I.The permeability of non-electro-lytes at high concentration. Biochimica et biophysica acta 59:35–46
    [Google Scholar]
  22. Van Zoelen E.J.J., Van Der Neut-Kok E.C.M., De Gier J., Van Deenen L.L.M. 1975; Osmotic behavior of Acholeplasma laidlawii B cells with membrane lipids in liquid-crystalline and gel state. Biochimica et biophysica acta 394:463–469
    [Google Scholar]
  23. West I.C. 1970; Lactose transport coupled to proton movements in Escherichia coli.. Bio-chemical and Biophysical Research Communica-tions 41:655–661
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-105-2-233
Loading
/content/journal/micro/10.1099/00221287-105-2-233
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error