1887

Abstract

Reduction of cytochrome from both horse heart and by FeSO has been demonstrated. This reaction was stimulated by nalidixic acid and structurally related compounds, and the effect was more pronounced for the yeast cytochrome. Divalent metal ions other than Fe lessened or abolished the stimulation by these compounds. Fe and other metal ions altered the spectra of nalidixic acid and related compounds indicating the formation of metal chelate complexes. 1,10-Phenanthroline inhibited reduction of cytochrome by Fe. Other divalent metal ions relieved the inhibition, probably by forming chelates with 1, 10-phenanthroline. These results suggest that metal ion chelation may be involved in the molecular mode of action of nalidixic acid and related drugs. The relevance of this artificial election transfer system to bacterial electron transfer is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-105-2-227
1978-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/105/2/mic-105-2-227.html?itemId=/content/journal/micro/10.1099/00221287-105-2-227&mimeType=html&fmt=ahah

References

  1. Albert A. 1958; Metal-binding agents in chemotherapy: the activation of metals by chelation. In The Strategy of Chemotherapy pp. 112–138 Edited by Cowan S.T., Rowatt E. London: Cambridge University Press.;
    [Google Scholar]
  2. Banič S. 1975; Synergistic antagonistic action of cysteine and iron compounds on the activity of spiramycin. Abstracts of the 9th International Congress of Chemotherapy, London.
    [Google Scholar]
  3. Carnevali F., Sarcoe L.E., Whittaker P.A. 1976; Differential effects of nalidixate on the cell growth of respiratory competent strains and cytoplasmic petite mutants of Saccharomyces cerevisiae. Molecular and General Genetics 146:95–100
    [Google Scholar]
  4. Dunkle L.D., Van Etten J.L., Bramble R.M. 1972; Mitochondrial DNA synthesis during fungal spore germination. Archiv für Mikrobiologie 85:225–232
    [Google Scholar]
  5. Dye W.E. 1956; An agar diffusion method for studying the bacteriostatic action of combinations of antimicrobial agents. Antibiotics Annual374–382
    [Google Scholar]
  6. Goss W.A., Deitz W.H., Cook T.M. 1964; Mechanism of action of nalidixic acid on Escherichia coli. Journal of Bacteriology 88:1112–1118
    [Google Scholar]
  7. Goss W.A., Deitz W.H., ClOOK T.M. 1965; Mechanism of action of nalidixic acid on Escherichia coli. II.Inhibition of deoxyribonucleic acid synthesis. Journal of Bacteriology 89:1068–1074
    [Google Scholar]
  8. Irving H., Williams R.J.P. 1948; Order of stability of metal complexes. Nature; London: 162746–747
    [Google Scholar]
  9. Kaminsky D., Meltzer R.I. 1968; Quinoline antibacterial agents.Oxolinic acid and related compounds. Journal of Medicinal Chemistry 11:160–163
    [Google Scholar]
  10. Lesher G.Y., Froelich E.J., Gruett M.D., Bailey J.H., Brundage R.P. 1962; 1,8-Naphthyridine derivatives: a new class of chemotherapeutic agents. Journal of Medicinal and Pharmaceutical Chemistry 5:1063–1065
    [Google Scholar]
  11. Mellor D.P., Maley L. 1948; Order of stability of metal complexes. Nature; London: 161436–437
    [Google Scholar]
  12. Minami S., Shono T., Matsumoto J. 1971; Pyrido [2,3-d]pyrimidine antibacterial agents.II.Piromidic acid and related compounds. Chemical and Pharmaceutical Bulletin, Tokyo 19:1426–1432
    [Google Scholar]
  13. Minami S., Yamabe S., Sakurai H., Hirose T. 1976; Synthesis of new pyrido-indole carboxylic acid derivatives. Japanese Patent Application Disclosure51–136698
    [Google Scholar]
  14. Shimizu M., Nakamura S., Takase Y. 1971; Piromidic acid, a new antibacterial agent: antibacterial properties. Antimicrobial Agents and Chemotherapy 1970:117–122
    [Google Scholar]
  15. Shimizu M., Takase Y., Nakamura S., Katae H., Minami A., Nakata K., Inoue S., Ishiyama M., Kubo Y. 1975; Pipemidic acid, a new antibacterial agent active against Pseudomonas aeruginosa: in vitro properties. Antimicrobial Agents and Chemotherapy 8:132–138
    [Google Scholar]
  16. Sillén L.G., Martell A.E. 1964 Stability Constants of Metal-Ion Complexes. Special Publication No.17 London: The Chemical Society;
    [Google Scholar]
  17. Yamabe S. 1976; Cytochrome c reduction by nalidixic and piromidic acids. Journal of Antimicrobial Chemotherapy 2:299–305
    [Google Scholar]
  18. Yamabe S., Shimizu M. 1977; Combination effects of chemotherapeutic agents.I.Antagonistic effects of 1,10-phenanthroline with nalidixic acid and piromidic acid on the growth of E.coli. Journal of Antimicrobial Chemotherapy 3:101–104
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-105-2-227
Loading
/content/journal/micro/10.1099/00221287-105-2-227
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error