1887

Abstract

Summary: A sp. and a sp. possessing crossing substrate saturation curves for -lactate were isolated from fresh water by chemostat enrichment. Their and values for -lactate were: sp., 23 m and 0·35 h, respectively; sp., 91 and 0·64 h, respectively. Under -lactate limitation, sp. outgrew sp. at dilution rates () above 0·29 h, but the converse occurred at lower values. The advantage of sp. increased with decreasing until, at = 0·05 h (i.e. -lactate concentration of approximately 1 ), sp. was eliminated from the culture essentially as a non-growing population. In sp. the for -lactate transport (5·8 ) was threefold lower than in sp. (20 ); sp. also possessed a higher for the transport of this substrate. The surface to volume ratio was higher in sp. and increased more markedly than in sp. in response to decreasing . Thus, a more efficient scavenging capacity contributes to the advantage of sp. at low concentrations of the carbon source. Although most of the enzymes of -lactate catabolism were more active in sp., NADH oxidase activity was about twice as high in sp.; and, unlike sp., the cytochrome content of this bacterium increased markedly with decreasing . A more active and/or more efficient respiratory chain may therefore also play a role in the advantage of sp. The other factors which appear to be involved include a lower energy of maintenance of sp. [0·016 g -lactate (g cell dry wt) h compared with 0·066 in sp.] and a lower minimal growth rate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-105-2-187
1978-04-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/105/2/mic-105-2-187.html?itemId=/content/journal/micro/10.1099/00221287-105-2-187&mimeType=html&fmt=ahah

References

  1. Calcott P.H., Postgate J.R. 1972; On substrate-accelerated death in Klebsiella aerogenes. Journal of General Microbiology 70:115–122
    [Google Scholar]
  2. Dean A.C.R. 1972; Influence of environment on the control of enzyme synthesis. Journal of Applied Chemistry and Biotechnology 22:245–259
    [Google Scholar]
  3. Duursma E.K. 1961; Dissolved organic carbon, nitrogen and phosphorus in the sea. Netherlands Journal of Sea Research 1:1–148
    [Google Scholar]
  4. Harder W., Kuenen J.G., Matin A. 1977; Microbial selection in continuous culture. Journal of Applied Bacteriology 43:1–24
    [Google Scholar]
  5. Hohorst H.F. 1963 In Methods of Enzymatic Analysis p. 266 Edited by Bergmeyer H.U. New York: Academic Press;
    [Google Scholar]
  6. Horiuchi T., Tomizawa J., Novick A. 1962; Isolation and properties of bacteria capable of high rates of β-galactosidase synthesis. Biochimica et biophysica acta 55:152–163
    [Google Scholar]
  7. Jannasch H.W. 1967; Enrichment of aquatic bacteria in continuous culture. Archiv für Mikrobiologie 59:165–173
    [Google Scholar]
  8. Jannasch H.W. 1969; Estimation of bacterial growth rates in natural waters. Journal of Bacteriology 99:156–160
    [Google Scholar]
  9. Kuenen J.G., Boonstra J., Schröder H.G.J., Veldkamp H. 1977; Competition for inorganic substrates among chemoorganotrophic and chemolithotrophic bacteria. Microbial Ecology 3:119–130
    [Google Scholar]
  10. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. Matin A., Gottschal J.C. 1976; Influence of dilution rate on NAD(P) and NAD(P)H concentrations and ratios in a Pseudomonas sp.grown in continuous culture. Journal of General Microbiology 94:333–341
    [Google Scholar]
  12. Matin A., Konings W.N. 1973; Transport of lactate and succinate by membrane vesicles of Escherichia coli, Bacillus subtilis and a Pseudomonas sp. European Journal of Biochemistry 34:58–67
    [Google Scholar]
  13. Matin A., Veldkamp H. 1974; Physiological basis of different substrate affinities of two fresh water organisms. American Society for Microbiology, Abstracts p. 50 Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  14. Matin A., Grootjans A., Hogenhuis H. 1976; Influence of dilution rate on enzymes of intermediary metabolism in two freshwater bacteria grown in continuous culture. Journal of General Microbiology 94:323–332
    [Google Scholar]
  15. Munro H.N., Fleck A. 1966; The determination of nucleic acids. Methods of Biochemical Analysis XIV:113–117
    [Google Scholar]
  16. Pirt S.J. 1972; Prospects and problems in continuous flow culture of microorganisms. Journal of Applied Chemistry and Biotechnology 22:55–64
    [Google Scholar]
  17. Pirt S.J. 1975 Principles of Microbe and Cell Cultivation. New York: John Wiley;
    [Google Scholar]
  18. Postgate J.R. 1969; Viable counts and viability. Methods in Microbiology 1:611–628
    [Google Scholar]
  19. Rigby P.W.J., Burleigh B.D.Jr Hartley B.S. 1974; Gene duplication in experimental enzyme evolution. Nature, London 251:200–204
    [Google Scholar]
  20. Smith R.W., Dean A.C.R. 1972; β-Galactosidase synthesis in Klebsiella aerogenes growing in continuous culture. Journal of General Microbiology 72:37–47
    [Google Scholar]
  21. Tempest D.W., Herbert D., Phipps P.J. 1967; Studies on the growth of Aerobacter aerogenes at low dilution rates in a chemostat. In Microbial Physiology and Continuous Culture pp. 240–254 London: H.M.S.O.;
    [Google Scholar]
  22. Veldkamp H. 1976 Continuous Culture in Microbial Physiology and Ecology. Durham: Meadowfield Press;
    [Google Scholar]
  23. Veldkamp H., Jannasch H.W. 1972; Mixed culture studies with the chemostat. Journal of Applied Chemistry and Biotechnology 22:105–123
    [Google Scholar]
  24. Vishniac W., Santer M.C. 1957; The thiobacilli. Bacteriological Reviews 21:195–213
    [Google Scholar]
  25. Wirsen C.O., Jannasch H.W. 1970; Growth response of Spirosoma sp.to temperature shifts in continuous culture. Bacteriological Proceedings 32:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-105-2-187
Loading
/content/journal/micro/10.1099/00221287-105-2-187
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error