1887

Abstract

The colourless alga survived carbon-starvation for at least 110 d. Over a period of 21 d total numbers of organisms and viable organisms declined by 62% and 68% respectively. Both acetate-supported and endogenous respiration rates decreased, and the values for adenylate charge decreased from 0·95 to 0·015 after 5 d starvation. Complex changes in respiratory enzymes, catalase and acid hydrolases occurred over a 27 d period; cytochromes b and c were modified to protohaem-like and haem c-like compounds, and no haem a derivative could be detected. Ultrastructural changes included indentation of cell walls, shrinkage of the cytoplasmic mass, and disorganization of mitochondrial inner membranes, although recognizable mitochondrial profiles were still present after 43 d.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-105-1-1
1978-03-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/105/1/mic-105-1-1.html?itemId=/content/journal/micro/10.1099/00221287-105-1-1&mimeType=html&fmt=ahah

References

  1. Atkinson D. E., Walton G. M. 1967; Aenosine triphosphate conservation in metabolic regulation. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  2. Ball W. J.Jr Atkinson D. E.Jr 1975; Adenylate energy charge in Saccharomyces cerevisiae during starvation. Journal of Bacteriology 121:975–982
    [Google Scholar]
  3. Bertini F., Brandes D., Buetow D. E. 1965; Increased acid hydrolase activity during carbon starvation in Euglena gracilis. Biochimica et biophysica acta 107:171–173
    [Google Scholar]
  4. Blum J. J., Buetow D. E. 1963; Biochemical changes during acetate deprivation and repletion in Euglena. Experimental Cell Research 29:407–421
    [Google Scholar]
  5. Brandes D., Buetow D. E., Bertini F., Malkoff D. E. 1964; Roles of lysosomes in cellular lytic processes. I. Effect of carbon starvation in Euglena gracilis. Experimental and Molecular Pathology (supplement) 3:583–609
    [Google Scholar]
  6. Callely A. G., Lloyd D. 1964; The metabolism of acetate in the colourless alga,Prototheca zopfii. Biochemical Journal 90:483–489
    [Google Scholar]
  7. Cartledge T. G., Lloyd D. 1972; Subcellular fractionation by differential and zonal centrifugation of aerobically grown glucose derepressed Saccharomyces cerevisiae. Biochemical Journal 126:381–393
    [Google Scholar]
  8. Chapman A. G., Fall L., Atkinson D. E. 1971; Adenosine triphosphate and synchronous mitosis in Physarum polycephalum. Journal of Bacteriology 96:330–337
    [Google Scholar]
  9. Chung C. W., Nickerson W. J. 1954; Polysaccharide synthesis in growing yeasts. Journal of Biological Chemistry 208:395–407
    [Google Scholar]
  10. Edwards C., Statham M., Lloyd D. 1975; The preparation of large-scale synchronous cultures of the trypanosomatid, Crithidia fasciculata, by cell-size selection: changes in respiration and adenylate charge through the cell cycle. Journal of General Microbiology 88:141–152
    [Google Scholar]
  11. Edwards S. W., Lloyd D. 1977; Changes in oxygen uptake rates, enzyme activities, cytochrome amounts and adenine nucleotide pool levels during growth of Acanthamoeba castellanii in batch culture. Journal of General Microbiology 102:135–144
    [Google Scholar]
  12. Edwards S. W., Chagla A. H., Griffiths A. J., Lloyd D. 1977; The cytochromes of Acanthamoeba castellanii. Biochemical Journal 168:113–121
    [Google Scholar]
  13. Elliott A. M., Bak I. J. 1964; The fate of mitochondria during ageing in Tetrahymena pyriformis. Journal of Cell Biology 20:113–129
    [Google Scholar]
  14. Huggins C., Lapides J. 1947; Chromogenic substrates. IV. Acyl esters of p-nitrophenol as substrates for the colorimetric determination of esterase. Journal of Biological Chemistry 170:467–482
    [Google Scholar]
  15. Hughes D. E. 1951; A press for disrupting bacteria and other micro-organisms. British Journal of Experimental Pathology 32:97–100
    [Google Scholar]
  16. John P. C. L., Thurston C. F., Syrett P. J. 1970; Disappearance of isocitrate lyase from cells of Chlorella pyrenoidosa. Biochemical Journal 119:913–919
    [Google Scholar]
  17. Katsunuma T., Schott E., Elsasser S., Holzer H. 1972; Purification and properties of tryptophan-synthetase-inactivating enzymes from yeast. European Journal of Biochemistry 27:520–526
    [Google Scholar]
  18. Levy M. R., Elliott A. H. 1968; Biochemical and ultrastructural changes in Tetrahymena pyriformis during starvation. Journal of Protozoology 15:208–222
    [Google Scholar]
  19. Levy M. R., Sisskin E. E., McConkey C. L. 1976; A protease that increases during a period of enzymic and metabolic adjustment in Tetrahymena. Archives of Biochemistry and Biophysics 111:634–647
    [Google Scholar]
  20. Lloyd D. 1974a Mitochondria of Microorganisms. London, New York: Academic Press;
    [Google Scholar]
  21. Lloyd D. 1974b; Dark respiration. In Algal Physiology and Biochemistry pp. 505–529 Stewart W. D. P. Edited by Oxford: Blackwells Scientific Publications.;
    [Google Scholar]
  22. Lloyd D., Brookman J. S. G. 1967; An oxygen electrode reaction vessel. Biotechnology and Bioengineering 9:271–272
    [Google Scholar]
  23. Lloyd D., Callely A. G. 1965; The assimilation of acetate and propionate by Prototheca zopfii. Biochemical Journal 97:176–179
    [Google Scholar]
  24. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  25. Luzikov N., Makhlis T. A., Galkin A. V. 1976; A probable role of cell proteinases in the biogenesis of mitochondria in yeast. FEBS Letters 69:108–110
    [Google Scholar]
  26. Miura T., Yanagita T. 1972; Cellular senescence in yeast caused by carbon-source starvation. I. Changes in activities of respiratory system and lipid peroxidation activity. Journal of Biochemistry 72:141–148
    [Google Scholar]
  27. Morris I., Syrett P. J. 1965; The effect of nitrogen starvation on the activity of nitrate reductase and other enzymes in Chlorella. Journal of General Microbiology 38:21–28
    [Google Scholar]
  28. Müller M., Baudhuin P., De Duve C. 1966; Lysosomes on Tetrahymena pyriformis. I. Some properties and lysosomal location of acid hydrolases. Journal of Cellular Physiology 68:165–175
    [Google Scholar]
  29. Ryan F. J. 1959; Bacterial mutation in a stationary phase and the question of cell turnover. Journal of General Microbiology 21:530–549
    [Google Scholar]
  30. Stickland L. H. 1951; The determination of small quantities of bacteria by means of the biuret reaction. Journal of General Microbiology 5:698–703
    [Google Scholar]
  31. Trinci A. P. J., Thurston C. F. 1976; Transition to the non-growing state in eukaryotic microorganisms. Symposia of the Society of General Microbiology 26:55–79
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-105-1-1
Loading
/content/journal/micro/10.1099/00221287-105-1-1
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error