SUMMARY: can accommodate to the presence of toxic levels of Cd. This adaptation can also be induced, though to a lesser degree, by pre-growth of the yeast in 50 µM-Zn. Growth of Cd-adapted yeast through several passages in Cd-free medium leads to a progressive decrease in Cd-tolerance by the yeast, suggesting that the adaptation did not involve selection of a Cd-resistant mutant. Chromatography on Sephadex G-75 of the soluble fraction from Cd-adapted yeast indicated that no metallothionein-like protein was present. This suggests that the mechanism of adaptation is unlike that of the higher eukaryotes.


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