Phylogenetic and epidemiological relatedness among transferable plasmids belonging to the IncC, IncM and IncH incompatibility groups has been studied by DNA-DNA filter hybridization. Hybridization was carried out on nitrocellulose microfilters, at low temperature, in formamide and under paraffin oil. The degree of hybridization among plasmids belonging to the IncC and IncM groups supported the conclusions drawn from genetic classification. Studies on relatedness among plasmids belonging to the IncH group allowed their classification into three phylogenetic sub-groups. Comparison of DNA sequences of three plasmids sharing the same genetic properties and isolated from different bacterial species suggested an epidemiological spread of the same plasmid.
AndersonE. S., HumphreysG. O., WillshawA.1975; The molecular relatedness of R factors in enterobacteria of human and animal origin. Journal of General Microbiology 91:376–382
BuissiereJ., CoynaultC., Le MinorL.1977; Etude des conditions d’expression du caractère raffinose chez E. coli et Salmonella. Annales de Microbiologie (Institut Pasteur) 128A:167–183
ChabbertY. A., GerbaudG. R.1974; Surveillance épidémiologique des plasmides respon-sables de la résistance au chloramphénicol de Salmonella typhi. Annales de Microbiologie(Institut Pasteur) 125A:153–166
ChabbertY. A., ScavizziM. R., WitchitzJ. L., GerbaudG. R., BouanchaudD. H.1972; Incompatibility groups and the classification of Fi− resistance factors. Journal of Bacteriology 112:666–675
IngramL. C., RichmondM. H., SykesR. B.1973; Molecular characterization of the R factors implicated in the carbenicillin resistance of a sequence of Pseudomonas aeruginosa strains isolated from burns. Antimicrobial Agents and Chemotherapy 3:279–288
KourilskyPH., LeidnerJ., TremblayG. Y.1971; DNA-DNA hybridization on filters at low temperature in the presence of formamide or urea. Biochimie 53:1111–1114
KourilskyPH., MercereauO., GrosD., TremblayG. Y.1974; Hybridization on filters with competition DNA in the liquid phase in a standard and a micro-assay. Biochimie 56:1215–1221
Le MinorL., CoynaultC., ChabbertY. A., GerbaudG. R., Le MinorS.1976; Groupes de compatibilité de plasmides métaboliques. Annales de Microbiologie(Institut Pasteur) 127B:31–40
RahalK., GerbaudG. R., ChabbertY. A.1973; Caractérization d’un facteur de résistance transférable de Vibrio cholerae biotype el tor. Annales de Microbiologie (Institut Pasteur) 124B:283–294
ScavizziM. R.1973; Nouveaux groupes d’incompatibilité des plasmides. Intérêt dans les épidémies de crêche à Escherichia coli 0111 :B4. Annales de Microbiologie (Institut Pasteu) 124B:153–167
ShatkinA. J.1969; Colorimetric reactions of DNA, RNA and protein determination. In Fundamental Techniques in Virology22 pp. 231–237HabelK., SalzmanN. P. Edited by New York and London: Academic Press.;
SmithH. R., GrindleyN. D. F., HumphreysG. O., AndersonE. S.1973; Interactions of group H resistance factors with the F factor. Journal of Bacteriology 115:623–628
WitchitzJ. L., ChabbertY. A.1972; Résistance transférable à la gentamicine. II. Transmission et liaisons du caractère de résistance. Annales de l’Institut Pasteur 122-3:367–378