Taxonomy and Epidemiology of Gram-negative Bacterial Plasmids Studied by DNA-DNA Filter Hybridization in Formamide Free

Abstract

Phylogenetic and epidemiological relatedness among transferable plasmids belonging to the IncC, IncM and IncH incompatibility groups has been studied by DNA-DNA filter hybridization. Hybridization was carried out on nitrocellulose microfilters, at low temperature, in formamide and under paraffin oil. The degree of hybridization among plasmids belonging to the IncC and IncM groups supported the conclusions drawn from genetic classification. Studies on relatedness among plasmids belonging to the IncH group allowed their classification into three phylogenetic sub-groups. Comparison of DNA sequences of three plasmids sharing the same genetic properties and isolated from different bacterial species suggested an epidemiological spread of the same plasmid.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-104-2-269
1978-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/104/2/mic-104-2-269.html?itemId=/content/journal/micro/10.1099/00221287-104-2-269&mimeType=html&fmt=ahah

References

  1. Anderson E. S., Humphreys G. O., Willshaw A. 1975; The molecular relatedness of R factors in enterobacteria of human and animal origin. Journal of General Microbiology 91:376–382
    [Google Scholar]
  2. Buissiere J., Coynault C., Le Minor L. 1977; Etude des conditions d’expression du caractère raffinose chez E. coli et Salmonella. Annales de Microbiologie (Institut Pasteur) 128A:167–183
    [Google Scholar]
  3. Chabbert Y. A., Gerbaud G. R. 1974; Surveillance épidémiologique des plasmides respon-sables de la résistance au chloramphénicol de Salmonella typhi. Annales de Microbiologie(Institut Pasteur) 125A:153–166
    [Google Scholar]
  4. Chabbert Y. A., Scavizzi M. R., Witchitz J. L., Gerbaud G. R., Bouanchaud D. H. 1972; Incompatibility groups and the classification of Fi resistance factors. Journal of Bacteriology 112:666–675
    [Google Scholar]
  5. Clowes R. C., Hayes W. 1968 Experiments in Microbial Genetics. Oxford and Edinburgh: Blackwell Scientific Publications.;
    [Google Scholar]
  6. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri. Journal of General Microbiology 72:543–552
    [Google Scholar]
  7. Datta N., Hedges R. W. 1971; Compatibility groups among Fi R factors. Nature; London: 234222–223
    [Google Scholar]
  8. Denhardt D. T. 1966; A membrane filter technique for detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  9. Falkow S. 1975 Infectious Multiple Drug Resistance. London: Pion.;
    [Google Scholar]
  10. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. Journal of Molecular Biology 12:829–842
    [Google Scholar]
  11. Grindley N. D. F., Grindley J. N., Anderson E. S. 1972; R factor compatibility groups. Molecular and General Genetics 119:287–297
    [Google Scholar]
  12. Grindley N. D. F., Humphreys G. O., Anderson E. S. 1973; Molecular studies of R factor compatibility groups. Journal of Bacteriology 115:387–398
    [Google Scholar]
  13. Guerry P., Falkow S. 1971; Polynucleotide sequence relationships among some bacterial plasmids. Journal of Bacteriology 107:372–374
    [Google Scholar]
  14. Hedges R. W., Datta N. 1971; Fi R factors giving chloramphenicol resistance. Nature; London: 234220–221
    [Google Scholar]
  15. Ingram L. C., Richmond M. H., Sykes R. B. 1973; Molecular characterization of the R factors implicated in the carbenicillin resistance of a sequence of Pseudomonas aeruginosa strains isolated from burns. Antimicrobial Agents and Chemotherapy 3:279–288
    [Google Scholar]
  16. Kourilsky PH., Leidner J., Tremblay G. Y. 1971; DNA-DNA hybridization on filters at low temperature in the presence of formamide or urea. Biochimie 53:1111–1114
    [Google Scholar]
  17. Kourilsky PH., Mercereau O., Gros D., Tremblay G. Y. 1974; Hybridization on filters with competition DNA in the liquid phase in a standard and a micro-assay. Biochimie 56:1215–1221
    [Google Scholar]
  18. Le Minor L., Coynault C., Chabbert Y. A., Gerbaud G. R., Le Minor S. 1976; Groupes de compatibilité de plasmides métaboliques. Annales de Microbiologie(Institut Pasteur) 127B:31–40
    [Google Scholar]
  19. Novick R. P., Clowes R. C., Cohen S. N., Curtiss R.III Datta N., Falkow S. 1976; Uniform nomenclature for bacterial plasmids: a proposal. Bacteriological Reviews 40:168–189
    [Google Scholar]
  20. Rahal K., Gerbaud G. R., Chabbert Y. A. 1973; Caractérization d’un facteur de résistance transférable de Vibrio cholerae biotype el tor. Annales de Microbiologie (Institut Pasteur) 124B:283–294
    [Google Scholar]
  21. Scavizzi M. R. 1973; Nouveaux groupes d’incompatibilité des plasmides. Intérêt dans les épidémies de crêche à Escherichia coli 0111 :B4. Annales de Microbiologie (Institut Pasteu) 124B:153–167
    [Google Scholar]
  22. Shatkin A. J. 1969; Colorimetric reactions of DNA, RNA and protein determination. In Fundamental Techniques in Virology 22 pp. 231–237 Habel K., Salzman N. P. Edited by New York and London: Academic Press.;
    [Google Scholar]
  23. Smith H. R., Grindley N. D. F., Humphreys G. O., Anderson E. S. 1973; Interactions of group H resistance factors with the F factor. Journal of Bacteriology 115:623–628
    [Google Scholar]
  24. Witchitz J. L. 1975; Résistance enzymatiques aux aminosides et aminosides semi-synthétiques. Médicine et Maladies Infectieuses 5–6bis:319–330
    [Google Scholar]
  25. Witchitz J. L. 1977 Applications épidémiologiques de la classification des plasmides par incompatibilité. Thèse de Biologie Humaine; Paris.:
    [Google Scholar]
  26. Witchitz J. L., Chabbert Y. A. 1971; Résistance transférable à la gentamicine. I. Expression de la résistance. Annales de l’Institut Pasteur 121:733–742
    [Google Scholar]
  27. Witchitz J. L., Chabbert Y. A. 1972; Résistance transférable à la gentamicine. II. Transmission et liaisons du caractère de résistance. Annales de l’Institut Pasteur 122-3:367–378
    [Google Scholar]
  28. Witchitz J. L., Gerbaud G. R. 1972; Classification de plasmides conférant la résistance à la gentamicine. Annales de l’Institut Pasteur 123:333–339
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-104-2-269
Loading
/content/journal/micro/10.1099/00221287-104-2-269
Loading

Data & Media loading...

Most cited Most Cited RSS feed