1887

Abstract

Wild-type R plasmids of the P1 incompatibility group mediated the transfer of chromosomal genes in , but only at very low frequencies. Two P1 R plasmids, which had originally been selected in for enhanced donor properties, promoted much higher levels of gene transfer in . One of these, R68.45, was used for linkage mapping. All markers tested mapped on a single circular linkage group. Segments of donor chromosome up to at least one-seventh of its total length were transferred and integrated into the recipient chromosome.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-104-2-201
1978-02-01
2021-05-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/104/2/mic-104-2-201.html?itemId=/content/journal/micro/10.1099/00221287-104-2-201&mimeType=html&fmt=ahah

References

  1. Beringer J. E. 1974; R factor transfer in Rhizobium leguminosarum. Journal of General Microbiology 84:188–198
    [Google Scholar]
  2. Beringer J. E., Hopwood D. A. 1976; Chromosomal recombination and mapping in Rhizobium leguminosarum. . Nature; London: 264291–293
    [Google Scholar]
  3. Cooke M., Meynell E. 1969; Chromosomal transfer mediated by de-repressed R factors in F Escherichia coli K12. Genetical Research, Cambridge 14:79–87
    [Google Scholar]
  4. Curtiss R., Renshaw J. 1969; F+ strains of Escherichia coli K-12 defective in Hfr formation. Genetics 63:7–26
    [Google Scholar]
  5. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. 1971; Properties of an R factor from Pseudomonas aeruginosa. Journal of Bacteriology 108:1244–1249
    [Google Scholar]
  6. Dixon R., Cannon F. C., Postgate J. R. 1975; Properties of the R factor R144 drd3 in Klebsiella pneumoniae strain M5a1. Genetical Research, Cambridge 28:327–338
    [Google Scholar]
  7. Evenchik Z., Stacey K. A., Hayes W. 1969; Ultraviolet induction of chromosome transfer by autonomous sex factors in Escherichia coli. Journal of General Microbiology 56:1–14
    [Google Scholar]
  8. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Molecular and General Genetics 144:243–251
    [Google Scholar]
  9. Hayes W. 1968 The Genetics of Bacteria and their Viruses, 2nd edn.. Oxford & Edinburgh: Blackwell Scientific Publications.;
    [Google Scholar]
  10. Hedges R. W., Jacob A. E. 1974; Transposition of ampicillin resistance from RP4 to other replicons. Molecular and General Genetics 132:31–40
    [Google Scholar]
  11. Jacob A. E., Cresswell J. M., Hedges R. W., Coetzee J. N., Beringer J. E. 1976; Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4. Molecular and General Genetics 147:315–323
    [Google Scholar]
  12. Johnston A. W. B., Beringer J. E. 1975; Identification of Rhizobium strains in pea root nodules using genetic markers. Journal of General Microbiology 87:343–350
    [Google Scholar]
  13. Johnston A. W. B., Beringer J. E. 1977; Chromosomal recombination between Rhizobium species. Nature; London: 267611–613
    [Google Scholar]
  14. Kondorosi A., Kiss G. B., Sorrai T., Vincze E., Dansalvi Z. 1977; Circular linkage map of the Rhizobium meliloti chromosome. Nature; London: 268525–527
    [Google Scholar]
  15. Lacey G. H., Leary J. V. 1976; Plasmidmediated transmission of chromosomal genes in Pseudomonas glycinea. Genetical Research, Cambridge 27:363–368
    [Google Scholar]
  16. Meade H. M., Signer E. R. 1977; Genetic mapping of Rhizobium meliloti. Proceedings of the National Academy of Sciences of the United States of America 74:2076–2078
    [Google Scholar]
  17. Moody E. E. M., Hayes W. 1972; Chromosome transfer by autonomous transmissible plasmids: the role of the bacterial recombination (rec) system. Journal of Bacteriology 111:80–85
    [Google Scholar]
  18. Moody E. E. M., Runge R. 1972; The integration of autonomous transmissible plasmids into the chromosome of Escherichia coli K12. Genetical Research, Cambridge 19:181–186
    [Google Scholar]
  19. Olsen R. H., Shipley P. 1973; Host range and properties of the Pseudomonas aeruginosa R factor R1822. Journal of Bacteriology 113:772–780
    [Google Scholar]
  20. Siddiqi O., Fox M. S. 1973; Integration of donor DNA in bacterial conjugation. Journal of Molecular Biology 77:101–123
    [Google Scholar]
  21. Smith D. I., Gomez Lus R., Rubio Calvo M. C., Datta N., Jacob A. E., Hedges R. W. 1975; Third type of plasmid conferring gentamicin resistance in Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy 8:227–230
    [Google Scholar]
  22. Towner K. J., Vivian A. 1976; RP4-mediated conjugation in Acinetobacter calcoaceticus. Journal of General Microbiology 93:355–360
    [Google Scholar]
  23. Vogel H. J., Bonner D. M. 1956; Acetylornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-104-2-201
Loading
/content/journal/micro/10.1099/00221287-104-2-201
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error