SUMMARY: Wild-type R plasmids of the P1 incompatibility group mediated the transfer of chromosomal genes in but only at very low frequencies. Two P1 R plasmids, which had originally been selected in for enhanced donor properties, promoted much higher levels of gene transfer in One of these, R68.45, was used for linkage mapping. All markers tested mapped on a single circular linkage group. Segments of donor chromosome up to at least one-seventh of its total length were transferred and integrated into the recipient chromosome.


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